RESUMO
To evaluate the cytoprotective role of the Cyanacobalamin on the detrimental effects of heat -induced stress on splenic tissue. An Experimental study. Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi from January 2010 to April 2010. Forty five Albino rats [180-200 grams] were selected and divided into group A [Control], group B [Heat - induced] and group C [Protective] Each group was further subdivided into three subgroups, based on the period of the study. The animals of the subgroups B and C received heat and the temperature was set at 42 C for six hours daily. Group C [Cl, C2 and C3] animals were protected with Cyanocobalamin [BETOLVEX] at the dosage of 0.8 mg/ kg of body weight intraperitonealy, two hours before heat induction. Then animals were sacrificed according to their time duration, spleens were removed and fixed in alcoholic formalin for 24 hours .Then tissue were processed in ascending strengths of alcohol from 70 to 100%,cleared in xylene, infiltrated and embedded in paraffin. 5 micron thick sections were stained with haemotoxylin and eosin and e. Blood samples were collected in EDTA K3 containing vacutainers for the hormonal assay of plasma ACTH level. in group B animal's heat-stress alters the immunoarchitecture of the spleen; size of all comportments of the white pulp reduces with the appearance of the tingitble body macsophages and individual apoptotic cells and free apoptotic bodies. This group also shows significant increase in plasma ACTH levels. Whereas the splenic architecture and plasma ACTH level in group C animals return to normal by the cytoprotective effects of the Cyanocobalamin. The current study demonstrates the beneficial effects of the Cyanocobalamin to alleviate the detrimental effects of the heat-stress on the immune organs
RESUMO
Administration of quinolone therapy is controversial during growing age as stated by earlier authors. The flouroquinolones are currently not indicated for young children because of arthropathy and adverse effect as new born shown by studies. However the effects of ciprofloxacin and ZnCl[2] on prenatal conceptus have remained undocumented. The present study was designed to compare the effects on conceptus after maternal ingestion of ciprofloxacin and ZnCl[2] using Wastar albino rats. Ciprofloxacin and ZnCl[2] was administrated to pregnant female albino rats. Ciprofloxacin with a dose of 20 mg/Kg bodyweight and ZnCl[2] 120 microg/100 gm bodyweight two times therapeutic dose for 10 days [from day 8-18 of pregnancy]. Each animal was weighted on day 1, day 8 and day 18 of pregnancy. Abortion resulted on day 18[th] of pregnancy. Each group of pregnant animals were sacrificed on day 18 of gestation by over dose of either anaesthesia, abdomen opened, uterus and both cornua containing conceptus identified, removed, there weight recorded, crown rump length was measured and was compared with similar value of control animals. The results were statistically analysed to find out the significance. The ciprofloxacin induces a mordanting effect as obviated by increased basophilia. Our study reveals that ciprofloxacin administered in maternal, decreased maternal body weight to 38.4 +/- 0.9 gm. However simultaneous ZnCl[2] maintained the body weight to 41.4 +/- 0.7 gm, while ZnCl[2] increased the body weight to 46.5 +/- 2.25 gm. The body weight and Crown Rump length [CR Length] in conceptus decreased by 4.52 +/- 0.10 gm and 3.06 +/- 0.09 Cm respectively. That ciprofloxacin and ZnCl[2] administration maintained the body weight and CR length by 5.46 +/- 0.09 gm and 3.79 +/- 0.13 Cm respectively. That ZnCl[2] administration increased the body weight and CR length by 6.71 +/- 0.05 gm and 4.15 +/- 0.08 Cm respectively. Prenatal administration of Ciprofloxacin caused reduction in growth rate and CR length, and ZnCl[2] maintained body weight and CR length and growth of the rat conceptus.