Study of Prostate Specific Antigen, Alkaline Phosphatase and Vitamin B12 in Prostate Cancer

Objective: This study aimed to find the relative risks of prostate cancer incidence associated with screening by serum prostate-specific antigen (PSA), alkaline phosphatase (ALP) and vitamin B12 levels.Methods: Serum samples of 488 subjects above 40 years withouttreatment or puncture/rectal examination were screened for PSA levels using Chemiluminescence. Prostate cancer cases and control groups were analysed for vitamin B12 using Chemiluminescence and alkaline phosphatase using fully automated chemistry analyser. The relation of ALP and Vitamin B12 to levels of PSA in prostate cancer was examined.Results: Incidence rate for the screening on the risk of prostate cancer by PSA levels and metastasis involvementwas significant. Out of 488 screened subjects, 347 were within normal range according to age reference values, 30 were showing prostate cancer, and 111 were with the high risk of metastasis. Results of the case-control analysis showed a positive correlation among ALP and PSA levels and negative with vitamin B12.

Is There Really A Need Of Vacuum Tubes In Blood Specimen Collection?

Introduction – Pre–analytical phase consists of large proportion of laboratory errors. Proper blood collection and timely processing are critical and first pre–analytical steps required for integrity of laboratory results. The influence of blood collection devices on laboratory tests is often overlooked. In this review, we aim to study the effect of blood collection devices on biochemistry test results. Materials and method – All samples examined were collected from patients that had been referred to laboratories for various clinical chemistry assays. In total 100 patient’s samples were monitored at our institute. Venous blood samples were collected of the same patient using two different modes 1) In vacuum tubes 2) In non–vacuum tubes Parameters analysed were LFT (SGOT, SGPT, ALP, Bilirubin) KFT (urea, creatinine) in plain tubes and Blood Glucose in fluoride tubes. Results and Conclusion – It was observed that there was no significant difference in the results of biochemical parameters analysed from vacuum and non–vacuum tubes. The p–value was not significant. (p>0.05). Thus although incidence of hemolysis, contamination etc. are less in vacuum tubes, non–vacuum tubes have their own advantage of being cost–effective which can be favourable for a government setup where patient load is huge.