RESUMO
The present study aimed to compare the morphometric measurements of Mangalarga Marchador horses of batida and picada marcha. Twenty-two linear and eight angular measurements of 222 males (130 of batida marcha and 92 of picada marcha) and 266 females (168 of batida marcha and 98 picada marcha) were compared in a completely randomized design, consisting of two treatments: horses of batida and picada marcha. The results were submitted to analysis of variance and means were compared by Fisher test (P<0.05). It was concluded that most of the measures of Mangalarga Marchador horses of batida and picada marcha have similar values, however, there are differences between some angles of members...
Assuntos
Animais , Masculino , Feminino , Fenômenos Biomecânicos , Biometria , Cavalos/anatomia & histologia , Marcha/fisiologia , Amplitude de Movimento Articular/fisiologia , Movimento/fisiologiaRESUMO
Dos cepas de C.neoformans var. gattii serotipo B, fueron aisladas en el Parque de los Patricios de la ciudad de Buenos Aires, Argentina. Las muestras se recogieron en primavera, mediante el hisopado de la superficie interior de huecos y grietas de Eucalyptus spp. y otros árboles que, lamentablemente, no fueron identificados en el momento de la recolección. Los hisopos se inocularon en placas de agar ácido cafeico suplementado con antibióticos que se incubaron a 28§C durante una semana con observación diaria. Los aislamientos produjeron colonias características de la especie, lo que permitió detectarlos y separarlos de otros microorganismos presentes en la muestra. La identificación se realizó mediante estudios de fermentación de carbohidratos, asimilación de fuentes de carbono y nitrógeno y detección de ureasa y fenoloxidasa. La variedad se determinó utilizando el medio agarizado de canavanina-glicina-azul de bromotimol (CGB). La caracterización genotípica por reacción en cadena de la polimerasa con primer aleatorio (AP-PCR con el primer (GACA)4)mostró que el patrón de bandas de ambos aislamientos se diferenciaba en una única banda. En ambos casos el material provino de especies arbóreas diferentes de Eucalyptus spp. Estos dos aislamientos de fuentes naturales demuestran que C.neoformans var. gattii está presente en el medio ambiente de la ciudad de Buenos Aires, no necesariamente asociado a Eucalyptus spp. Este hallazgo podría explicar su asociación esporádica a infecciones humanas en la población local.
Assuntos
Poluição Ambiental/análise , Cryptococcus neoformans , ArgentinaRESUMO
The aim of our work was to determine the genotypic relatedness among H. capsulatum isolates from Argentinian patients with or without AIDS and isolates from other sources and geographic origins. We analyzed band patterns of 16 isolates obtained by RAPD-PCR, 11 Argentinian clinical isolates (8 from patients with AIDS and 3 from patients without AIDS), 2 strains from the CDC, 2 isolates from Mexico and one isolate from Uruguay. With the band patterns obtained, we built a dendrogram, which showed 3 major groups. Group I contained 2 isolates from Mexico, group II 1 strain from CDC and group III included 13 isolates, and was divided into 4 subgroups. Subgroup IIIa contained 2 isolates from patients with and without AIDS. Subgroup IIIb included isolates from patients without AIDS from Chaco. Subgroup IIIc contained 7 isolates from Argentina (from Buenos Aires province) and the Uruguayan isolate (6 isolates from patients with AIDS). Subgroup IIId included only one strain from CDC. All the Argentinian and the Uruguayan isolates went into group III, subgroups IIIa, IIIb and IIIc. This is a preliminary study; however, a relatedness of the RAPD-PCR band patterns and the geographic origin was observed. Although there might be a probable association between immunocompromised patients with AIDS and infective capability of some H. capsulatum isolates, further studies with a larger number of isolates from different sources and geographic origins are necessary to confirm these results.
Assuntos
Animais , Humanos , Histoplasma , Histoplasmose , Técnica de Amplificação ao Acaso de DNA Polimórfico , Argentina , Histoplasma , Histoplasmose , Hospedeiro Imunocomprometido , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/microbiologia , México , Modelos Biológicos , Filogenia , Reação em Cadeia da Polimerase , Quirópteros/parasitologia , UruguaiRESUMO
Dermatomycoses are an important sanitary problem. Our country counts with partial epidemiological data only referred to a few geographical areas. The aim of our work was to perform a multicenter study with the participation of 15 medical centers located at the Capital City and in 11 Provinces of Argentina with different socioeconomical and cultural characteristics. Between October and December 1998, 851 ambulatory patients of both sexes (58 female, 42 male) with lesions compatible with mycoses in skin, nails or scalp were studied. Only 51.6 of the patients had mycoses confirmed by direct microscopic examination and/ or culture. Dermatophytes caused 69.3 of the diagnosticated infections, yeast 12.2 and Malassezia furfur 13.3. Agents of dermatomycoses most frequently isolated were T. rubrum (52.7), T. mentagrophytes (19.2) and M. canis (14.2). C. albicans was the predominant causative agent of yeast infections (44.4). In children below 11 years old, the most frequent infections were tinea capitis 55.4 (M. canis 60, T. tonsurans and M. gypseum 8.3) and tinea corporis 20 (M. canis 46, T. mentagrophytes 30). The most frequent localizations in adult were toenail and feet (63) (T. rubrum 47.3 and 45.7, T. mentagrophytes 9.5 and 23.4, respectively), fingernail 15.6 (C. albicans 25 and T. rubrum 8.3) and trunk and extremities 12.2 (M. furfur 57.5 and T. mentagrophytes 14.9). Direct examination allowed the diagnosis of 94.66 of all positive samples and 91.74 of the cases with positive isolation.