The expression of Th1/Th2 type cytokines and clinical significance in Uyghur cervical cancer / 实用肿瘤学杂志

Objective To assess the immune level of cervical cancer patients and precancerous lesions patients,and investigate the association of Th 1 /Th2 type cytokines expression and clinical pathologic factors in the plasma of Uyghur cervical cancer patients .Methods we collected peripheral blood specimens from cervical cancer patients,precancerous lesion(CINⅢ)patients and controls.The expressions of Th1 type cytokines IL-2, IFN-γand Th2 type cytokines IL-4,IL-10 in plasma were detected by ELISA .Receiver operating characteris-tic curve( ROC curve) was used to analyze the cytokines value of auxiliary diagnosis in cervical cancer .Results The results showed that compared with control group ,the expressions of Th1 type cytokines IL-2,IFN-γin cer-vical cancer and precancerous lesion group were significantly reduced (P<0.05).The expressions of Th2 type cytokines IL -4, IL -10 in cervical cancer and precancerous lesion group were significantly increased ( P <0.05).in cervical cancer group,IL -10 expression gradually increased with tumor pathological staging (P <0.05).IL-2 expression level in Uyghur cervical cancer patients was significantly lower than in Han patients (P<0.05).Compared cervical cancer group with control group ,AUC of IL-2,IFN-γ,IL-4 and IL-10 were 0.979,0.766,0.736 and 0.903.Conclusion Cellular immune level of cervical cancer patients was low and Th1/Th2 shift has occurred , which suggests that it may be one of the mechanisms in immune escape of tumor cells.Th1 /Th2 type cytokines detection has a certain significance for auxiliary diagnosis of cervical cancer .Be-sides,the decrease of IL-2 expression may play an important role in the occurrence and development of Uyghur patients with cervical cancer .

Association of defective HLA-I expression with antigen processing machinery and their association with clinicopathological characteristics in Kazak patients with esophageal cancer / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>It has been confirmed that defective expression of human leukocyte antigen class I (HLA-I) molecules can contribute to the immune evasion of cancer cells in some types of cancer. The aim of this study was to examine the expression of HLA class I antigen and the antigen-processing machinery (APM) components in esophageal squamous cell carcinoma (ESCC) and their role in high risk human papillomavirus (HPV) infection, and to analyze their association with histopathological characteristics in the Kazak ethnic group.</p><p><b>METHODS</b>A total of 50 formalin-fixed, paraffin-embedded ESCC lesions were collected from the First Affiliated Hospital of Xinjiang Medical University, China. The expression levels of HLA-I antigen and APM components were determined by immunohistochemistry; the HPV DNA were detected using polymerase chain reaction (PCR).</p><p><b>RESULTS</b>A high frequency of down-regulation or loss of expression of HLA and APM components were found in esophageal cancer in Kazak people. HLA-I, TAP1, CNX, LMP7, Erp57, Tapasin and ERAP1 were down-regulated in 68%, 44%, 48%, 40%, 52%, 32% and 20% of ESCC lesions then, respectively. The loss of expression of HLA-I antigen was significantly correlated with part of the APM components and positively correlated with high risk HPV16 infection. TAP1, CNX, LMP7, Erp57 and Tapasin loss were significantly associated with tumor grading, lymph node metastasis and depth of invasion (P < 0.05).</p><p><b>CONCLUSION</b>Our results suggest that APM component defects are a mechanism underlying HLA-I antigen down-regulation in ESCC lesions, and indicate that the loss expression of HLA-I and APM components will become an important marker of ESCC and analysis of HLA-I and APM component expression can provide useful prognostic information for patients with ESCC from the Kazak ethnic group.</p>

An improved method to recover high quality genomic DNA from paraffin-embedded tissues / 中南大学学报(医学版)

Objective To establish a high quality genomic DNA preparation method from formalin fixed and paraffin embedded tissues (FFPET) by integrating previous DNA extraction methods. Methods We combined classical genomic DNA extraction methods and commercially available DNA affinity column, replaced the de-waxing by dimethylbenzene with water-bath, designed a fast and improved genomic DNA preparation method. We also extracted genomic DNA from paraffin embedded cervical cancer tissues, and checked the quality of DNA by agarose gel electrophoresis and polymerase chain reaction detection. Results The improved genomic DNA extraction method combined the advantages of the water-bath de-waxing and DNA affinity column, making it possible to get high quality genomic DNA from paraffin embedded cervical cancer tissues, and especially efficient to recover genomic DNA fragments larger than 20 kb. Conclusion The improved DNA extraction method is fast and convenient to recover high quality genomic DNA from paraffin embedded tissues.

Two-dimentional liquid phase chromatographic proteome profiles in Uighur women with uterine cervix cancer / 中南大学学报(医学版)

Objective To establish differential protein expression profile specific for serum proteome of cervical carcinoma in Uighur women by protein 2-dimensional liquid phase chromatogra-phy. Methods We collected and prepared sera of cervical carcinoma from Uighur women, and sepa-rated and processed data using 2-dimensional liquid phase chromatography system specific for protein (ProteomeLabTM PF-2D ). Results A differential expression profile of serum proteome based on characteristics of protein isoelectrie point and hydrophobic features was successfully established. Fifty-six differentially expressed protein spots were found, among which there was an obvious difference in 12 peaks. Conclusion Two-dimensional liquid phase chromatography is an effective and feasible method to establish differential expression profile of serum proteome and to offer a new way for the screening of serum protein markers of Uighur women with cervical carcinoma.