1.
Journal of the Egyptian Society of Parasitology. 2013; 43 (2): 419-423
em Inglês
| IMEMR
| ID: emr-170619
RESUMO
This paper describes a simple technique for staining of flatworms using lactophenol cotton blue [LPCB]. The staining was tested on 2 trematode species: Heterophyes heterophyes and Mesostephanus appendiculatus, and one cestode: Diplopylidium acanthotetra, which were collected from the intestine of stray cats in Kuwait. The specimens were mounted in a small amount of the LPCB stain on a clean slide for 2-3 minutes before covering with a cover slip. The technique rapidly and clearly differentiated the internal structures of the helminthes. Its speed and simplicity are advantages over other staining methods. It is easily used in wide-scale surveys where a large number of platyhelminths have to be identified and it is suitable for field studies