Q fever [coxlella burnet II] lamong man and farm animals in North Sinai, Egypt

Antibodies against Coxiella burnetii were estimated among sheep, goats and camels [190], their owners [150 patients with pyrexia of unknown origin] and 30 normal individuals in North Sinai over the 2006 by indirect immounofluorescence assay. Nested polymerase chain reaction was used to detect Com-1 gene [genetic target of C. burnetii] encoding a 27-kDa outer membrane protein in the samples. C. burnetii IFA antibodies [IgM and IgG] in patients were 8 [5.3%] and a healthy control [3.3%]. The overall was 9 of 180 [5.0%]. C. burnetii IgM were detected in 3/150 [2%] patients with positive genome, while IgG were detected in 5/150 patients, only the three who had IgM and IgG had positive genome suffered high fever. C. burnetii antibodies were detected in 20 [22.5%], 12 [16.8%] and 4 [13.3%] of sheep, goats, camels, which total 36/190 [18.9%]. The positive genome of these IFA positive animals was 10 [50.0%], 4 [33.3%] and zero [0.0%] respectively. On the other hand, Rhi-picephalus sanguineus [dog tick] and Dermacentor andersoni [wood tick] were identified on some Q fever infected animals. The results were discussed

Natural infection of fresh and brackish water fish with heterophyid encysted metacercariae in Dakahlia Governorate Egypt

The encysted metacercariae [EMC] of heterophyids in the fish species; Mugil caphalus, M. capito, M. auratus, Oreochromis, n.niloticus [=Tilapia nilotica], and Tilapia zillii trapped brackish and fresh water fishes were isolated by the artificial digestion method and identified. The EMC by morphometric analysis proved to be seven phenotypical distinct types. Cross-matched seven groups of clean laboratory bred puppies each was experimentally infected with a distinct EMC type. The recovered adult flukes were compatible to seven species of six genera of family Heterophyidae. These were Heterophyes heterophyes, H. aequalis, Pygidiopsis genata, Haplorchis yokogawai, Prohemostomum vivax, Phagicola sp. and Stictodora sp. The EMC of the seven heterophyids were recovered from all the brackish water fishes, M. caphalus, M. capito, M. auratus, O. niloticus and T. zillii. On the other hand, the EMC of P. genata, Haplorchis yokogawai and Phagicola sp. were recovered only from the fresh water fish, O. niloticus and T. zillii. All the EMC was re-described and photographed

study on the expulsion of trichinella spiralis from the small intestine of immunocompetent and immunosuppressed albino rats

This study was carried out on 70 Albino rats classified into 3 groups. Group I, of 10 rats [control], Group II, of 30 rats infected with T. spiralis, Group III, of 30 rats, infected with T. spiralis and immunosuppressed with dexamethasone. Rats were sacrificed on the 8th,15th and 21th days postinfection. At each day of sacrification, histological examination, adult worms count, goblet cells and mucosal mast cells count were done. The results showed that in GII there were shortening of villi, prominent lengthening of the crypts, high significant increase of goblet cells and mucosal mast cells numbers during worms expulsion and most of adult worms were expelled at 15th day post infection. GIII, showed marked atrophy of the villi and crypts, high significant decrease of goblet cells and mucosal mast cells numbers and significant delay of adult worms expulsion. The authors concluded that goblet cells and mucosal mast cells play an important role in T. spiralis expulsion and immunosuppression led to dalay expulsion of T. spiralis