RESUMO
Production of reactive oxygen species (ROS) yields serious damage oxidation of proteins, lipids and genomic structures. Studies have shown that production of ROS increases during intensive exercise training. This study aimed to investigate the effect of high intensity interval training on 8-oxoguanine DNA glycosylase (OGG1) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in the brain and liver of rats. 16 adult Albino Wistar rats were randomly divided into sedentary control and high intensity interval training groups. Animals ran on treadmill for 6 weeks, 6 days per week, at 95 to 100 percent of maximal oxygen consumption. Using commercial kits, the content of OGG1 and 8-OHdG were measured using sandwich ELISA assay. Data analyzed using Student’s T-test at P≤0.05 level. High intensity interval training resulted in significant increases in contents of OGG1 in brain (t14=7.22, P=0.001) and liver (t14=2.55, P=0.02) of rats. However, high intensity interval training had no significant influence on 8-OHdG levels in brain (t14=1.60, P=0.13) and liver (t14=1.28, P=0.22) of rats. Also, there were no significant differences between changes in the brain and liver contents of OGG1 (t14=0.97, P=0.34) and 8-OHdG (t14=0.42, P=0.68) of rats following high intensity interval training. Taken together, separation of the training sessions to various bouts of exercise with maximum effort, through increase in OGG1 contents, will lead to modify of 8-OHdG levels in brain and liver.
RESUMO
Objectives: Diabetes mellitus is now seen as a worldwide epidemic disease with prevalent and incidence data. Exercise training is known to promote beneficial changes in diabetic patient.Materials & Methods: A number of 60 male rats weighing 180 to 310 grams, 13 weeks old were divided into six groups. The exercise protocol was aerobic training for six weeks. In this study, factors like Insulin resistance, glucose transporter type 4 and AMP-activated protein kinase were measured. The data were analyzed using one-way analysis of variance test in P< 0.05 level. Results: The results showed a significant difference in insulin resistance (P=0.001, F5, 37= 10.80), Glucose transporter type 4 (P=0.001, F5, 37= 20.14) and AMP-activated protein kinase (F5, 37 = 16.2, P= 0.001) levels among different groups. There was no significant difference between the impact of continuous and interval training on these indices (P=1.00).Conclusions: The continuous and interval training by increasing the Glucose transporter type4 protein content and AMP-activated protein kinase lead to reduced blood glucose levels and improved insulin resistance. Furthermore, we could use conducting interval training as a therapeutic approach to manage diabetes for the participants who were not able to perform the continuous training due to fatigue.