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1.
Asian Pac J Allergy Immunol ; 2004 Jun-Sep; 22(2-3): 153-8
Artigo em Inglês | IMSEAR | ID: sea-37157

RESUMO

Scleroderma is an enigmatic rheumatic disorder of uncertain etio-pathogenesis. Cancer has an approximately two-fold higher incidence in scleroderma patients than in the general population. There are preliminary data of acquired genetic damage in scleroderma but the significance of these observations are uncertain. To determine somatic mutation frequency at the glycophorin-A (GPA) locus in patients with limited and diffuse cutaneous scleroderma. The GPA assay measures the total somatic mutation frequency (Vf), composed of gene inactivating mutations (NO) and mutations arising from mitotic recombination (NN) in individuals heterozygous for the GPA MN blood group. Mutation frequency was determined using a validated GPA flow cytometric assay using fluorescent labeled monoclonal antibodies specific for the GPA blood groups M and N. This assay detects and enumerates progeny of red blood cell (rbc) precursor cells which have acquired genetic damage resulting in a loss of expression of one of the GPA alleles. It was found that patients with scleroderma (n = 23) had significantly elevated Vf as compared with young healthy controls (p < 0.001) and elderly controls (p = 0.03). Patients with diffuse scleroderma had higher mean Vf as compared with limited scleroderma (p = 0.055). In comparison with controls, patients with scleroderma exhibit a higher proportion of mitotic recombinant mutations than inactivating mutations (p < 0.002). There was no correlation between Vf and disease duration, age at onset or autoantibody status. We have documented evidence of acquired genetic damage at the GPA locus in scleroderma. Evidence of acquired genetic damage in this disorder may be importance in explaining both the etio-pathogenesis of scleroderma and the association of scleroderma with cancer.


Assuntos
Adolescente , Idoso , Alelos , Feminino , Citometria de Fluxo , Instabilidade Genômica , Glicoforinas/genética , Humanos , Masculino , Pessoa de Meia-Idade , Mutação , Escleroderma Sistêmico/genética
2.
Asian Pac J Allergy Immunol ; 2001 Dec; 19(4): 275-82
Artigo em Inglês | IMSEAR | ID: sea-37074

RESUMO

Immunohistochemical, flow cytometric and ELISA studies were performed to examine the expression of endoglin (CD105, a TGF beta receptor) on dermal endothelial cells, peripheral blood monocytes and free and bound serum levels in patients with systemic sclerosis as compared with appropriate controls. Endoglin was found to be significantly upregulated on dermal blood vessels in patients with scleroderma (and in patients with inflammatory skin disorders) as compared to healthy skin (p < 0.05). In contrast, there was no significant difference in endoglin expression on circulating blood monocytes between scleroderma patients and patients with a rheumatic disoder or healthy control subjects; however, endoglin expression was upregulated on monocytes in inflammatory joint fluid from patients with rheumatoid arthritis. Endoglin expression on monocytes was also influenced by isolation techniques and during whole blood culture. No differences were found in circulating free or bound endoglin levels between scleroderma patients and healthy controls. In conclusion, endoglin expression on dermal endothelial cells was significantly enhanced in scleroderma but levels on circulating monocytes and in the serum were within normal limits. The functional significance of this upregulation is uncertain but may reflect endothelial activation in scleroderma.


Assuntos
Idoso , Idoso de 80 Anos ou mais , Antígenos CD , Células Cultivadas , Derme/citologia , Endotélio/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Fibrose/fisiopatologia , Citometria de Fluxo , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Monócitos/metabolismo , Receptores de Superfície Celular , Receptores de Fatores de Crescimento Transformadores beta/sangue , Escleroderma Sistêmico/fisiopatologia , Telangiectasia/fisiopatologia , Molécula 1 de Adesão de Célula Vascular/sangue
3.
Asian Pac J Allergy Immunol ; 1998 Jun-Sep; 16(2-3): 81-6
Artigo em Inglês | IMSEAR | ID: sea-36432

RESUMO

Approximately 20% of patients with the limited form of scleroderma will develop pulmonary hypertension which is generally a late stage fatal complication. Why pulmonary hypertension occurs in this subset of patients is unknown and it has not been possible to predict which patients are at risk. Nailfold capillary dilatation, distortion and drop occurs universally in patients with scleroderma and is generally an early finding. The present study was conducted to investigate whether quantitative nailfold capillaroscopy could distinguish those limited scleroderma patients who have established pulmonary hypertension. Quantitative nailfold capillaroscopy was performed by Visual Image Analysis in 10 healthy subjects and 20 patients with limited scleroderma (18 centromere +ve), of whom 8 had established pulmonary hypertension. It was found that scleroderma patients with pulmonary hypertension had a significant reduction in capillary density compared with patients lacking this complication (p < 0.01). Patients with scleroderma have significantly more dilated capillaries than controls although no significant differences were observe between the two patient subgroups. The finding of reduced nailfold capillary density in scleroderma patients with established pulmonary hypertension has possible pathogenic significance and may allow detection of this subgroup at an early stage in their disease progression.


Assuntos
Adolescente , Adulto , Idoso , Capilares/patologia , Progressão da Doença , Feminino , Humanos , Hipertensão Pulmonar/diagnóstico , Processamento de Imagem Assistida por Computador , Masculino , Microscopia de Vídeo , Pessoa de Meia-Idade , Unhas/irrigação sanguínea , Esclerodermia Localizada/complicações , Escleroderma Sistêmico/complicações
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