Combination of salermide and cholera toxin b induce apoptosis in MCF-7 but not in MRC-5 cell lines

Sirtuin1 is an enzyme that deacetylates histones and several non-histone proteins including P53 during the stress. P300 is a member of the histone acetyl transferase family and enzyme that acetylates histones. Hereby, this study describes the potency combination of Salermide as a Sirtuin1 inhibitor and cholera toxin B [CTB] as a P300 activator to induce apoptosis Michigan Cancer Foundation-7 [MCF-7] and MRC-5. Cells were cultured and treated with a combination of Salermide and CTB respectively at concentrations of 80.56 and 85.43 micro mol/L based on inhibitory concentration 50 indexes at different times. The percentage of apoptotic cells were measured by flow cytometry. Real-time polymerase chain reaction was performed to estimate the messenger ribonucleic acid expression of Sirtuin1 and P300 in cells. Enzyme linked immunosorbent assay and Bradford protein techniques were used to detect the endogenous levels of total and acetylated P53 protein generated in both cell lines. Our findings indicated that the combination of two drugs could effectively induced apoptosis in MCF-7 significantly higher than MRC-5. We showed that expression of Sirtuin1 and P300 was dramatically down-regulated with increasing time by the combination of Salermide and CTB treatment in MCF-7, but not MRC-5. The acetylated and total P53 protein levels were increased more in MCF-7 than MRC-5 with incubated combination of drugs at different times. Combination of CTB and Salermide in 72 h through decreasing expression of Sirtuin1 and P300 genes induced acetylation of P53 protein and consequently showed the most apoptosis in MCF-7 cells, but it could be well-tolerated in MRC-5. Therefore, combination of drugs could be used as an anticancer agent

Study of relationship between different kinds of finger print and blood pressure in infected and non-infected patients afflicted with blood pressure, hospitalized at Ali Ebn Abitaleb Hospital in Rafsanjan, in second half of 2002

Conceding the wide effect of high blood pressure on different organs of body and its different complications and as this disease doesn't have any signs and people don't aware about their dieses when they are affected by finger print and modifying the association between that and finger print, it is possible to diagnose the disease on them and take the right treatments. In this research which was case-control research we collected fingerprints from 100 persons that were at Ali-Ebn-Abitaleb Hospital and 100 normal persons that the same paper is used and characteristic in two group classified in 3 categories: whirl Arch loop and from the SPSS and t-test method was made for statistic analysis. Results showed from 2000 samples of finger print that were taken from 100 persons that had blood pressure and 100 persons that didn 't have blood pressure, the average loop fingerprint in blood pressure group was 3.82 +/- 2.27 and in non blood pressure group 6.5 +/- 1.81 that has the significance deterrence [P value=0.023]. The average Whirl fingerprint in blood pressure group was 4.73 +/- 2.59 and in none blood pressure group was 2.29 +/- 1.93 that has the significances difference [P value= 0.002] and the average arch finger print in blood pressure group was 1.43 +/- 1.55 and in none blood pressure group was 1.04+0.93 that has the significance difference. [P value=0.0001]. Among fingerprints and high blood pressure are relationships and on the basis of the resolutions of research possibility of taking to high blood pressure in people that have whirl fingerprint is more