The Epidermal Mucus Extract Of Clarias Batrachus Inhibits Gram Negative Bacteria Growth / Jurnal Sains Kesihatan Malaysia

Recently, epidermal mucus of fish has been identified as a rich biological source of various bioactive substances,particularly antibacterial peptides. This study was conducted to identify the potential of epidermal mucosa of Clariasbatrachus as a source of antibacterial material. Mucosal samples were collected from C. batrachus epidermis and extractedusing an aqueous extraction method. Antibacterial activity of the crude epidermal mucus extract was determined byusing spectrophotometric method. As a result there was antibacterial activity by the mucus extract against Escherichiacoli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). The results of this study supports the role of mucus asa part of body immune system with antibacterial peptide as a component of innate body immune system. Hence, thesefindings can be used as one of the first steps towards the development of new class antibiotics.

Identification of Gram Positive and Negative Bacteria Microflora Isolated from Hot Water Spring in Bentong, Pahang / Jurnal Sains Kesihatan Malaysia

A study on the isolation and identification of bacterial flora from hot spring was carried out from Bentong hot springwater, Pahang. In general, many of the bacteria identified from the hot spring were from Bacillus sp. due to its abilityto adapt to high temperature mainly by forming endospore. The objective of this study was to identify bacteria otherthan Bacillus sp. that were able to survive this hot spring. Three hot spring pools were identified as A, B and C. Thetemperatures were ranging from 36°C to 52°C and their pH values were between 7.88-8.65. The result shows thattwo species of Gram positive cocci and three species of Gram negatives were isolated. The two Gram positives wereStreptococcus sp. and Staphylococcus sp. The three species of Gram negative rods were identified as Pseudomonas sp.,Klebsiella sp. and Proteus sp. In conclusion, non-sporulating species of bacteria other than Bacillus sp. have adaptedto survive in hot spring

Prevalence Study of Candidiasis by Conventional Pap Smear at Sarawak General Hospital, Malaysia / Jurnal Sains Kesihatan Malaysia

This study was conducted to determine the prevalence of candidiasis among women of 20 to 59 years of age at Sarawak General Hospital by examining the conventional Pap smear. The prevalence of this infection was studied among the five main ethnic groups comprising of Iban, Chinese, Malay, Bidayuh and Orang Ulu. Microscopic screening of 300 conventional Pap smears which had been processed and stained with the Papanicolaou staining method was conducted. Based on the information provided in the Pap smear screening form, the clinical manifestations were observed which may provide some indications on the type of infection. The details of the clinical manifestations were very important because in an infection or inflammation, Pap smears would usually demonstrate the cyto-morphological changes of the epithelial cells. The age factor among the different ethnics also plays important role in the assessment of the infection frequency. Candida sp. was detected in 12.7% of the specimens. The infection occurred in all ethnic groups as well as in all age groups. All cases presented with clinical signs and symptoms. The results of this study revealed the importance of undergoing Pap smear screening for candidiasis detection besides screening for the early detection of cervical cancer

Identification of Bacterial Flora other than Bacillus sp. from Bentong Hot Spring in Pahang, Malaysia / Jurnal Sains Kesihatan Malaysia

A study on the isolation and identification of bacterial flora from hot spring was carried out using water from Bentong hot spring in Pahang. In general most of the bacteria identified from the hot springs were from Bacillus sp. This was due to its ability to adapt to high temperature mainly by forming endospore. The objective of this study was to identify bacteria other than Bacillus that were able to survive in hot springs. Three hot spring pools were identified (A,B and C) with temperatures ranging from 36°C to 52°C and pH values between 7.88-8.65. This study has succesfully isolated two species of Gram positive cocci and three species of Gram negative bacilli. The two Gram positives were Streptococcus sp. and Staphylococcus sp. The three species of Gram negative rods were unidentified and were denoted as X, XI and XII.

Prevalence of Bacterial Vaginosis from Examination of Conventional Pap Smears at Sarawak / Jurnal Sains Kesihatan Malaysia

This study was conducted to determine the prevalence of bacterial vaginosis among women of age 20 to 59 years at Sarawak General Hospital by examining the conventional Pap smear. The prevalence of this infection was studied among the five main ethnic groups comprising the Iban, Chinese, Malay, Bidayuh and Orang Ulu. Microscopic screening of 300 conventional Pap smears which had been processed and stained with the Papanicolaou staining method was conducted. Based on the information provided in the Pap smear screening form, the clinical manifestations were observed which may provide some indications on the type of infection. The details of the clinical manifestations were very important because in an infection or inflammation, Pap smears would usually demonstrate changes on the cyto-morphology of the epithelial cells. The age factor among the different ethnics also played important role in the assessment of the infection frequency. Bacterial vaginosis was detected in 79.7% (239/300) of cases. The infection occurred in all ethnic groups and the infections were more frequently seen among the 20 to 39 years age group. Majority of the cases (93.9%) presented with clinical signs and symptoms. The results of this study revealed the importance of undergoing a Pap smear screening for bacterial vaginosis detection besides screening for the early detection of cervical cancer

Prevalence Study of Trichomonas vaginalis by Conventional Pap Smear at Sarawak General Hospital, Malaysia / Jurnal Sains Kesihatan Malaysia

This study was conducted to determine the prevalence of Trichomonas vaginalis among women of age 20 to 59 years at Sarawak General Hospital by examining the conventional Pap smear. The prevalence of this infection was studied among the five main ethnic groups comprising the Iban, Chinese, Malay, Bidayuh and Orang Ulu. Microscopic screening of 300 conventional Pap smears which had been processed and stained with the Papanicolaou staining method was conducted. Based on the information provided in the Pap smear screening form, the clinical manifestations were observed which may provide some indications on the kind of infection. The details of the clinical manifestations were very important because in an infection or inflammation, Pap smears would usually demonstrate changes on the cyto-morphology of the epithelial cells. The age factor among the different ethnics also played important role in the assessment of the infection frequency. Trichomonas vaginalis was detected in 7.7% (23/300) of the specimens. This infection occurred in all ethnic groups and the infections were more frequently seen among 20 to 29 years age group. All the cases presented with clinical signs and symptoms. The results of this study revealed the importance of undergoing Pap smear screening for Trichomonas vaginalis infection detection besides screening for the early detection of cervical cancer.

Antibacterial Activity of Rafflesia cantleyi Solms-Laubach Extracts against Gram Positive and Negative / Jurnal Sains Kesihatan Malaysia

Rafflesia cantleyi Solms-Laubach is a wild plant found in lowland Peninsular of Malaysia and used widely in traditional medicines. The main objective is to screen antibacterial activity of Rafflesia cantleyi extract. The plant was extracted with cold extraction involving three stages of extraction with solvents such as petroleum ether, ethyl acetate and finally ethanol. All extracts were tested against Staphylococcus aureus ATCC 29523, Staphylococcus epidermidis, Escherichia coli ATCC 25922 and Salmonella typhimurium using well diffusion method. As a result, ethyl acetate and methanol extract showed antibacterial inhibition against tested bacteria whereas petroleum ether failed to show any. Most bacteria are more susceptible to ethyl acetate compared to ethanol extract. The Minimum Inhibitory Concentration (MIC) MICrodilution test and minimum bactericidal concentration (MBC) was carried out on both of the extract. Ethyl acetate extract has MIC ranging between 6.25 – 12.5 mg/ml and MBC between 25.0 – 50.0 mg/ml. Ethanolic extract has much higher MIC and MBC value, which ranges between 25.0mg/ml to 50.0 mg/ml for it’s MIC and has the MBC of 100.0 mg/ml.