RESUMO
Objective: To assess the neuroprotective effects of local administration of 17- beta- estradiol on nerve regeneration
Methods: Sixty female Wistar rats were overiectomized and divided into four experimental groups [n = 15], randomly: In autograft group a segment of sciatic nerve was transected and re-implanted reversely. In sham-surgery group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group defect was bridged using a silicon conduit filled with 10 microL [0.1 mg/mL] 17- beta- estradiol. Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period
Results: Behavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in other groups [p<0.05]. Immunohistochemical reactions to S-100 in treatment group were more positive than that in other groups
Conclusion: Local administration of 17-beta-estradiol improved functional recovery and morphometric indices of sciatic nerve. It could have clinical implications for the surgical management of patients after facial nerve transection