RESUMO
The aim of this investigation was insight the potential health effects of Spirulina and its extracts, especially its effect on the treatment of cancer. The anticarcinoma effect induced by polysaccharide was evaluated in vitro using Ehrlich Ascites Carcinoma Cells [EACC] and Hepatic cell line phase G2 [HEPG2]. LC50 for both were 6.1 and 8.93 microg/ml respectively. And in vivo, Polysaccharide exhibited more potent antiproliferation activity against Ehrlich Ascites Carcinoma Cell [EACC] through intraperitoneal injection of five groups of female mice.The 1st group serves as control, the 2[nd] group injected one time by EACC at dose 5xl0[6] cells/body weight, the 3[rd] group injected with polysaccharide three times at dose [50 mg/body weight crude extract] which is equivalent to 6.1 microg/ml for each injection. The 4[th] group was injected firstly by EACC at dose 5xl06 cells/body weight and then polysaccharide was injected three times 2 days apart at dose [50 mg/body weight crude extract] for each injection. The 5[th] group was injected three times with polysaccharide then with EACC one time at the same dose. All animals were sacrificed after 10 days. Biochemical and histopathological. Our study revealed that in both groups of either injection of mice with polysaccharide before and after EACC, the activities of both ALT and AST were normalized. Moreover the dramatic effects on WBCs, RBCs, and Hb were improved, indicating that polysaccharide is hepatoprotectant through the inhibition of the proliferation of EACC. This was confirmed with histopathological investigation which revealed that, most of hepatocytes of female mice injected with polysaccharide three times, then injected with EACC appeared intact with wide central vein. The blood sinusoids between hepatic cords revealed many phagocytic Von Kupffer cells. Many hepatocytes showed division and other appeared normal