RESUMO
We explored the liver enzymes characteristics of dengue patients with fatty liver in Xishuangbanna,providing evi-dences for local dengue fever effective treatment program.The clinical data of 1 9 1 dengue patients with fatty liver and 1 9 1 ran-domized controls was retrospectively analyzed on the patients'mainly liver enzymes indicators in Xishuangbanna Prefecture from 2013 to 2015.Results showed that the 191 cases with fatty liver,the growth rate of such as alanine aminotransferase was 84.8%,aspartate aminotransferase was 90.1%,gamma glutamyltransferase was 76.8% and bilirubin index was 20.4%,except bilirubin index,the other 3 indicators in dengue patients with fatty liver were higher than those in the control group (P<0.05). The liver injury was serious for the dengue cases with fatty liver in Xishuangbanna,of those alanine aminotransferase,aspar-tate aminotransferase and gamma glutamyltransferase were significantly increased.These results suggested that doctors should pay attention to above 3 enzymes changes and take related treatments immediately as soon as dengue cases with fatty liver were detected.
RESUMO
<p><b>OBJECTIVE</b>To establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD).</p><p><b>METHODS</b>Based on the gene-specific region of the following pathogens: Chlamydia trachomatis omp1/ompb, herpes simplex virus (HSV) DNA polymerase, Treponema pallidum tpp47, Haemophilus ducreyi 16s rRNA, four sets of primers were designed and an M-PCR assay was developed to detect four pathogens in one test. The assay was evaluated with diagnostic result of golden standard for each pathogen.</p><p><b>RESULTS</b>Of the 51 clinical samples, M-PCR showed slightly higher positive rate (47.1%) of HSV than cell culture (23.6%). Meanwhile, the positive rate of T. pallidum detected by M-PCR and dark-field microscopy was 19.6% (10/51) and 15.7% (8/51), respectively. Only one sample was positive for H. ducreyi and no sample was positive for C. trachomatis detected by both M-PCR assay and culture.</p><p><b>CONCLUSION</b>This primary study indicated that M-PCR assay can simultaneously and rapidly detect the four etiologic pathogens causing GUD.</p>