Comparison of volatile constituents in two types of mugwort leaves (produced in Qichun and Nanyang) using the headspace GC-MS / 针灸推拿医学（英文版）

Objective:To compare the volatile constituents in mugwort leaves produced in Qichun, Hubei Province and Nanyang, Henan Province. Methods:The volatile constituents were extracted using headspace heating and analyzed using gas chromatography-mass spectrometry (GC-MS). Then a qualitative analysis was made according to the standard database provided by the National Institute of Standards and Technology (NIST) and the relative contents of each constituent were calculated using the peak area normalization method. Results:A total of 59 compounds were identified from the mugwort leaves from Qichun and 51 compounds were identified from the mugwort leaves from Nanyang. These mainly include monoterpenoids, sesquiterpenoids, CxHyOz and other compounds involving the aldehyde, ketone, alkane and benzene. The mugwort leaves from Qichun and Nanyang share 32 common volatile constituents. The chromatographic peak area of identified compounds accounting for 96.38% of GC-MS total chromatographic peak areain Qichun mugwort leaves, versus 95.54% of that in Nanyang mugwort leaves. Conclusion:The headspace heating extraction combined with GC-MS technology can evidently display similarities and differences of volatile constituents in mugwort leaves produced in different areas and thus provide scientific basis for the quality and screening of mugwort leaves.

Clinical investigation of treatment with Qinghuang powder for 86 patients with chronic myelogenous leukemia / 白血病·淋巴瘤

Objective To verify the clinical effect of Qinghuang powder in chronic myelogenous leukemia(CML). Methods 86 CML patients treated with Qinghuang powder, in which 28 cases also partially received herbal medicine (activating blood circulation to dissipate blood stasis). Results 62 cases had complete remission (72.1%), 14 cases partial remission (16.3 %), advance 8 cases(9.3 %), inefficiency 2 cases (2.3 %), and the total efficiency was 97.7 %. The symptom were improved after a week when patients had taken medicines. 44 cases have hepatomegalia, among them 39 cases have diminished or became normal compared to untreated. 70 cases have splenoparectasis, among them 60 cases became normal, 9 cases diminished, and 1 case had no change after treatment. It took 15.5 days in average when spleen began to diminish, and took 62.9 days to become smallest. The WBC began to decrease at 10.4 day and took 54.8 days in average became normal. The major side effect was digestive tract symptom, followed by skin pigmentation and skin excessive cornification. It could be avoided by low dosage. Conclusion Qinghuang powder could not only induce CML to CR, but also improve the clinical symptom of CML and eliminate the infiltration of leukemia cells. It has little influence to Hb and Plt.

Study on antiinflammatory effect of different chemotype of Cinnamomum camphora on rat arthritis model induced by Freund's adjuvant / 中国中药杂志

<p><b>OBJECTIVE</b>To study the antiinflammatory effects of naphtha from different chemotypes of Cinnamomum camphora and natural borneol on the rat arthritis model induced by Freund's adjuvant.</p><p><b>METHOD</b>The arthritis model was induced by injecting Freund's adjuvant in rat voix pedis dermis and the rats were randomly divided into seven groups: normal control group, model control group, triptergium wilfordii control group, borneol chemotype naphtha group, camphor chemotype naphtha group, isocamphane chemotype naphtha group and natural borneol group. Rats of the triptergium wilfordii control group were given orally 8.1 mg x kg(-1) triptergium wilfordii for 35 days, rats of the normal control group and model control group were given same volume water, and rats of other groups were given 80 mg x kg(-1) corresponding drug. We observed the rat common condition, weighed the rat body weight weekly, measured the degree of swelling of voix pedis every 4 days, weighed the thymus and spleen on the end of life, and measured the contents of cell factor TNF-alpha, IL-2, and IL-6 in rat blood serum.</p><p><b>RESULT</b>As far as the arthrosis degree of swelling and the contents of cell factor TNF-alpha, IL-2, IL-6 were concerned, rats of model control group were higher than normal control group, and rats of other drug groups were lower than the model control group. The order of inhibition ratios of the arthrosis degree of swelling from high to low principle was isocamphane chemotype naphtha group, camphor chemotype naphtha group, borneol chemotype naphtha group and natural borneol group. All medication administration teams evidently reduced the contents of the IL-2 and IL-6, and the inhibition ratios were higher than 38%. In the case of the contents of TNF-alpha and IL-2, all groups were not evidently different. In the case of inhibition of IL-6, camphor chemotype naphtha group was better than borneol chemotype naphtha group and natural borneol group, the latter was better than isocamphane chemotype naphtha group. As far as the weight, thymus index and spleen index were concerned, all medication administration groups were not different.</p><p><b>CONCLUSION</b>The different chemotypes of C. camphora have anti-inflammatory effect on the rat arthritis model induced by Freund's adjuvant, but pharmacological activity and mechanism of action are different. The study points out the clinical curative effects of the chemotypes of the kindred medicinal plant are different, and please consider the difference of chemotype in clinical application.</p>

Changes of adenyl cyclase and phosphodiesterase activities at different time point in hypothalamus of rats with fever and hypothermia / 中国病理生理杂志

AIM: To observe the changes of adengl cyclase(AC) and phosphodiesterase(PDE) activities of at different time point in hypothalamus of rats with fever and hypothermia. METHODS: Radioisotope method was used to measure the activity of AC and PDE. RESULTS:The fresh yeast caused rats fever after subcutaneous injection 4h( P