RESUMO
PON1 [Paraoxonase 1] is an esterase enzyme which is associated with high density lipoprotein [HDL]. The enzyme prevents the peroxidation of low density lipoprotein [LDL]. The susceptibility of LDL for oxidation is the proven risk factor for coronary artery diseases. The aim of this study is to survey the effect of atherogenic diet with or without enzyme inhibitors on the incidence and progression of atherosclerosis in rabbits. Twenty four New Zealand white rabbits divided into three groups [control, under the atherogenic diet, atherogenic diet and nandrolone decanoate - paraoxonase inhibitor therapy] and were treated for two months. At the beginning and end of the treatment, 5 mL of blood was obtained to determine the levels of total antioxidant capacity [TAC], HDL, mallon dialdehide [MDA] and PON1. After sixty days rabbits anesthetized under standard conditions, and sampling carried out from heart arteries for pathological examinations. Data were analyzed using statistical software SPSS/15 and one way ANOVA and paired t-test statistical tests. The results showed that the plasma levels of TAC, HDL, MDA and PON1 had significant changes in this study [P<0.05]. The pathological study showed that in the presence of PON1, the formation and progression of atheroma is diminished. The results of this study showed that cholesterol-rich diet decreased serum level of PON1 which in turn led to a reduction in formation and progression of atheroma. It was shown that the enzyme inhibitor helps accelerating the development of atheroma.
RESUMO
Staphylococcus aurous has been known as one of the most common nosocomial pathogens which include as much as 30% to 50% of general population. Regarding to the presence of carrier staff can be considered as one of the infection source; remarking of carrier for prevention purposes is important. In a cross-sectional study we determined the prevalence of staphylococcus aurous carriage among 113 edical staff of Madani Heart Hospital, Tabriz [Iran] 28/08/2009 to 28/09/2009 on [Physicians, nurses, technicians, secretaries, the administrative personnel and services], including surgical wards, laboratory, ICU and CCU. In this study, 113 participants comprised of 60 males [53%] and 53 females [47%], and their mean age of participants in this study was 34.8 years [20-55]. Sampling was carried out from nasal of employees. Samples were place on blood agar and mannitol salt agar; suspected colonies have been cultured and identified by catalase, coagulase and DNAase. Generally, of 113 people who entered the study, 30 person [26.5%] had positive cultures and 82 [72.6%] had a negative culture. The incidence of staphylococcus aureus carriage was more in men than women 57% vs. 43% [p<0.05]. In this study staphylococcus carriage incidence in Tabriz heart centre [Madani hospital] was 50%to 80% lower than our estimation, unexpectedly