Pharmacophoric screening of newly synthesized isoniazid derivatives and their antimycobacterial activity

Mycobacterium tuberculosis is clinically recognized as a causative agent of Tuberculosis. Keeping in view, this study was endeavored to screen our previously synthesized seventeen INH analogues for their antimycobacterial potential using proportion method. During this process, INH and all the seventeen compounds were examined at different concentrations of 0.05, 0.1 and 0.2microg/mL which were prepared using Lowenstein-Jensen [LJ] base. For drug susceptibility test, three Mycobacterial strains ATCC H37Rv, known INH-sensitive and INH-resistant strains were selected, sub-cultured on LJ Medium and serial diluted to achieve 1: 10, 1: 100, 1: 1000 and 1: 10000 from calibrated bacterial suspension Mcfarland No. 1. Dilutions of 1: 100 and 1: 10000 were added to drug free medium and 1: 100 bacterial suspension was added to each of the test concentrations and finally incubated for 4-6 weeks at 37 degree C. It was observed that only compounds II and XI were active against MTb. Compounds III, IX and X also showed activity but were less potent. Ligand Scout 3.02[il_10] was used to perform pharmacophore-based screening where important pharmacophoric features were identified in the structures of these compounds which could be related to their observed antimycobacterial activity

Feasible choices in diagnostic methods of malaria .

Background & objectives: The present study was undertaken to find out a new easy method in the diagnosis of malaria by centrifuged buffy coat smear, which was found to be a feasible and reasonable procedure. Methods: Blood samples collected from 120 patients suspected of malaria were subjected to all three diagnostic modalities—peripheral blood smear (PS), centrifuged buffy coat smear (CBCS) and antigen detection test using pLDH and aldolase (AG). Results: The results of various methods were compared. It was seen that addition of centrifugation (i.e. CBCS) to conventional method of PS (i.e. thick and thin smears) improved its sensitivity from 85 to 93.3%. Antigen detection and CBCS were found superior to PS in sensitivity. CBCS gives combined sensitivity and specificity of both antigen and PS. Conclusion: CBCS is as sensitive as antigen test and as specific as PS in species identification. It is a reasonable and feasible procedure too.