RESUMO
Background: the functional unil of the Kidney is the uriniferous tubule which consists of a nephron and a collecting duct. The nephron consists of two components – the renal corpuscle and the real tubule. Blood is filtered by the renal corpuscle and million renal corpuscles in each kidney. Renal diseases that involve the nephron mostly affect renal corpuscles. Due to aging there is gradual increase in size of renal corpuscle with subsequent decrease of renal function. It is essential to know the functional status of an aged kidney for management of kidney diseases or renal transplantation. As there are very few known studies about the diameter of renal corpuscle in Bangladeshi people we designed this study . Objective: To find out the different in diameters in renal corpuscles in micrometer in the stained section of kidneys in different age groups. Materials and Methods: This is an obsercational type of study carried out in the department of Anatomy of sir Salimullah Medical College (SSMC) from january 2004 to December 2005 the study was performed on50 pairs of human kidneys. All these samples were collected from the bodies of unclaimed autopsied individuals from the morgue of the departments of forensic Medicine of Dhaka Medical College and SSMC. The collected samples were divided into three groups- Group A (6-20yrs), B(21-35yrs) and Group C(36-65 yrs). Results; the diameters of renal corpuscles were found 157.10 ± 5.58 and 154.25±8.90 micrometers in the right and left kidneys of Group A, 159.04± and micrometers in the right and left kidneys of Groups B and 176.58 ± 6.69 and 178.35 ± 8.84 micrometers in the right and left kidneys of Group C. there was significant difference of diameters between Group A and Group C and between Group B and Group C. Conclusion: the diameter of renal Corpuscle increases significantly with age after 35 years
RESUMO
The crude methanolic extract of the bark of Sarcolobus globosus (Family-apocynaceae) and its different organic soluble Kupchan fractions were screened for total phenol content (TPC), cytotoxic, membrane stabilizing and thrombolytic activities. The polyphenol content was determined colorimetrically using Folin-Ciocalteu method and expressed in gallic acid equivalent. The chloroform soluble Kupchan fraction (CSF) exhibited higher level of Total Polyphenol Contents (TPC, 54.21 gm of GAE/100 gm of dried extract). In the brine shrimp lethality bioassay, the crude methanolic extract (MEBP) exhibited significant cytotoxicity. The membrane stabilizing activity was assessed by using erythrocyte in hypotonic solution and was compared with acetyl salicylic acid. The hexane soluble Kupchan fraction (HSF) produced 52.73 percent inhibition of hemolysis of RBC as compared to 65.38 percent revealed by acetyl salicylic acid (0.10 mg/mL). In thrombolytic study screening, the crude methanolic extract demonstrated significant thrombolytic activity in human blood specimen.
El extracto crudo metanólico de la corteza de Sarcolobus globosus (Familia-apocynaceae) y sus diferentes fracciones solubles Kupchan fueron identificadas para contenido total de fenoles (CTF), actividades citotóxicas, estabilizantes de membrana y trombolíticas. El contenido de polifenoles fue determinado colorimétricamente usando el método Folin-Ciocalteu y expresados en equivalentes a ácido gálico. La fracción Kupchan soluble en cloroformo (FSC) exhibió los mayores niveles de Contenido Total de Polifenoles (CFT, 54,21 gm of GAE/100gm de extracto seco). En el bioensayo de letalidad (Artemia salina), el extracto metanólico crudo (EMC) exhibió una siginificativa citotoxicidad. La actividad estabilizadora de membrana fue estimada usando eritrocitos en un medio hipotónico y fue comparado con el ácido acetil salicílico. La fracción Kupchan soluble en hexano (FSH) produjo un 52,73 por ciento de inhibición de la hemólisis de los glóbulos rojos comparado con un 65,38 por ciento revelado por el ácido acetil salicílico (0,1 mg/mL). En las determinaciones trombolíticas, el extracto metanólico crudo demostró una significativa actividad trombolítica en una muestra de sangre humana.