Qualitative and quantitative investigations of furocoumarin derivatives [Psoralens] of Haplophyllum tuberculatum [Rutaceae]

Haplophyllum tuberculatum is an indigenous plant widely distributed in Iraq. .Phytochemical investigation of this plant indicating that the plant is rich in alkaloids, fixed and volatik oils; but non about psoralen contents. Preliminary investigation indicated that this plant has furocoumarins in acceptable amount. Based on these results together with literature survey it was deemed desirable to carry out this phytochemical with emphasis on isolation and characterization of the furocoumarins compounds, Detailed preliminary investigation of the furocoumarins content of the dried, ground whole plant has been described and the results were discussed. Thin layer chromatography of the petroleum ether [boiling point 60-80degree C] extract demonstrated the occurance of furocoumarins compounds in the plant. The extraction procedure of the plant material, the fractionation and isolation procedures of the furocoumarins are fully described. One compound was isolated and identified as Ammoidin by TLC [compared with standard], melting point [148 C], mixed melting point [148 C] and HPLC. Spectrophotometer apparatus was used to determine exactly the amount of Ammoidin measured at 304nm at UV absorption. In Haplophyllum tuberculatum the maximum amount of Ammoidin was found sample collecting from Daquok during June.

Phytochemical investigation of Suaeda beccata [chenopodiaceae]

This work covers some phytochemical studies of a wild plant, Suaeda baccata Forsk of the family Chenopodiaceae, which covers almost all the cities of Iraq. Literature survey revealed a number of publications concerning different species of Suaeda, but none at all concerned Suaeda baccata. therefore, a research on this plant will be of important value. Several reports indicated the presence of alkaloids in some Suaeda species, thus our investigation procedures emphasized the identification and isolation of the alkaloids that might be present in this plant. Preliminary investigation on the plant extract showed the presence of the alkaloids that were tested by both Mayor's reagent and Dragendorffs spraying reagent, and authenticated with standard compounds by TLC using several solvent systems that proved the presence of two alkaloids, trigonelline and choline. Trigonelline crystals were subjected to tests of purity as HPLC, melting point and mixed melting point, which matches with the standard trigonelline. Extract containing choline alkaloid was identified only by TLC using different solvent systems. Studies concerning its crystallization and identification by HPLC and melting point were left for further studies since choline is highly hygroscopic and oxidizable compound

Anti-microbial investigation of Suaeda baccata [chenopodiaceae]

This study includes the detection of the anti microbial activity of both the plant extract of Suaeda baccata [Chenopodiaceae] and its constituent the trigonelline alkaloid, against several micro-organisms [Staphylococcus aureus to represent one member of Gram positive bacteria, Escherichia coli to represent one member of Gram negative bacteria and Candida albicans to represent one member of fungi] in that trigonelline alkaloid and the plant extract, showed an antibacterial activity against the growth of gram positive bacteria, Staphylococcus aureus, while gram negative bacteria, Escherichia coli and the yeast, Candida albicans were resistant to both