Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs

Mycoplasma (M.) hyosynoviae is known to colonize and cause disease in growing-finishing pigs. In this study, two clinical isolates of M. hyosynoviae were compared by inoculating cesarean-derived colostrum-deprived and specific-pathogen-free growing pigs. After intranasal or intravenous inoculation, the proportion and distribution pattern of clinical cases was compared in addition to the severity of lameness. Tonsils were found to be the primary site of colonization, while bacteremia was rarely detected prior to the observation of clinical signs. Regardless of the clinical isolate, route of inoculation, or volume of inocula, histopathological alterations and tissue invasion were detected in multiple joints, indicating an apparent lack of specific joint tropism. Acute disease was primarily observed 7 to 10 days post-inoculation. The variability in the severity of synovial microscopic lesions and pathogen detection in joint cavities suggests that the duration of joint infection may influence the diagnostic accuracy. In summary, these findings demonstrate that diagnosis of M. hyosynoviae-associated arthritis can be influenced by the clinical isolate, and provides a study platform to investigate the colonization and virulence potential of field isolates. This approach can be particularly relevant to auxiliate in surveillance and testing of therapeutic and/or vaccine candidates.

Anestesia intrduural y cirugía ambuattoria / Intradural anesthesia and ambulatory surgery

Se realizó un estudio transversal descriptivo a 190 pacientes sometidos a cirugía ambulatoria en la unidad quirúrgica del Hospital Clínico Quirúrgico Docente Amalia Simoni , desde abril de 2004 hasta de 2006.Las variables seleccionadas para el estudio fueron edad, sexo, patologías quirúrgicas e incidencia de la cefalea postpuncion dural. Predominó el sexo masculino (64.7 %) y el mayor porcentaje de los operados se encontraba entre los 36 y 46 años de edad, el diagnóstico operatorio mas frecuente fue la hernia inguinal para un 38.42. Consideramos que el uso de aguja espinal de 27 gauge con punta de lápiz por su incidencia mínima en la cefalea postpunción dural es recomendable para efectuar la anestesia espinal en la cirugía ambulatoria.

Descriptive cross-sectional study to 190 patients underwent to ambulatory surgery was carried out in the surgical unit at "Amalia Simoni" Surgical Clinical Teaching Hospital from April 2004 to April 2006. The selected variables for the study were the age, sex, surgical pathologies and incidence of headache dural postpuncture. Male sex predominated (64.7 %) and the major percentage of the operated was between 36 and 46 years-old, the most frequent operative diagnosis was the inguinal hernia for a 38.42%. We consider that the use of 27 gauge spinal needle with tip of pencil by its minimum incidence in the headache dural postpuncture is recommendable to perform the spinal anesthesia in the ambulatory surgery.