RESUMO
Association of genetic polymorphism in the calpastatin [CAST] gene with average daily gain was examined in Iranian purebred Kurdi sheep. The genotypes for CAST were determined by the PCR-SSCP method. Blood samples were collected from 84 purebred Kurdi sheep belonging to the Kurdi Breeding Station located in the Khorasan province, north-east of Iran. Extraction of genomic DNA was based on the Guanidinium Thiocyanate-Silica gel method. Three genotypes including aa, ab and ac with frequencies of 0.55, 0.32 and 0.13, were observed in this population. Chi-square test confirmed Hardy-Weinberg equilibrium for the CAST loci. Average heterozygosity [37%] of the CAST locus for Kurdi sheep was slightly low. Daily gain birth to weaning [GBW]; weaning to six months [GWS]; six to nine months [GSN] and nine to yearling [GNY] were analyzed by a statistical model comprising SSCP [Single strand conformation polymorphism] and significant effect [P<0.05] of CAST genotypes was observed for GBW only
RESUMO
To prevent distribution of recessive alleles in dairy herds all bulls used for AI [Artificial Insemination] have to be tested. In this study 26 blood and 4 semen samples were supplied from Iranian Holstein bulls used for AI. Genomic DNA was extracted from 100 ml of blood and 200 ml of semen. Samples were tested by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism [PCR-RFLP] method. PCR reaction was performed for amplification of polymorphic region of the CD18 gene on chromosome 1 and exon 5 of ASS gene on chromosome 11. We detected one Bovine Leukocyte Adhesion Deficiency [BLAD] carrier and no carrier for bovine Citrolliemia in this study. Hardy-Weinberg test confirmed the equilibrium of BLAD locus in this population