investigation on the effect of alcoholic and aqueous extracts of Dorema aucheri [Bilhar] on some pathogenic bacteria in vitro

Dorema aucheri is a plant that grows in Iran. In Persian it is called [Bilhar]. This experimental study was carried out at Ferdowsi University of Mashhad in 2014. After collection and preparation of aqueous and ethanolic extracts of Dorema aucheri [Bilhar], The antibacterial activity of ethanolic and aqueous extracts of Bilhar was evaluated against 7 laboratory strains of microorganisms, including 4 Gram positive [Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus and Bacillus subtilis] and 3 Gram negative bacteria [Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris]. Its effects against human pathogen microorganism were determined using "Spreading of the Extract on Medium Surface" and "Disk Agar Diffusion Method", Minimal Inhibitory Concentration [MIC] and Minimum Lethal Concentration [MLC] were determined for this extract. Collected data were analyzed by SPSS software using one-way ANOVA. The zone of inhibition for the ethanolic extract varied from 8 mm for P. aeruginosa to 24 mm for S. pyogenes and from 7 mm for P. aeruginosa to 19 mm for S.pyogenes in the aqueous extract. The minimum inhibitory concentration [MIC] of the extracts ranged between 2 mg/ml and 64 mg/ml while the minimum lethal concentration [MLC] ranged between 4 mg/ml and 256 mg/ml. Among of tested strains, P. aeruginosa has maximum MIC and MBC. 30 and 40 mg/mL Concentrations of Redcurrant have significant antimicrobial effect on bacteria. Antibacterial effect of extracts was decreased with decrease of extract concentration in disk. According to result, ethanolic extract of Dorema aucheri have antimicrobial effect on growth of all of the strains exposed analyzes and antimicrobial effect of that was maximum on Gram-positive bacterum of S. pyogenes. P. aeruginosa showed the highest level of resistance against the aqueous and ethanolic Bilhar extracts. The present study demonstrated that the ethanol leaf extract of Dorema aucheri hold an excellent potential as an antibacterial agent

Antimicrobial effect of Carboxy Methyl Cellulose [CMC] containing aqueous and ethanolic Eucalyptus camaldulensis L. leaves extract against Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis

Oil from the eucalyptus tree [Eucalyptus camaldulensis L.] is used today in many over the counter cough and cold products, to relieve congestion. Eucalyptus oil is also used in creams and ointments to relieve muscle and joint pain, and in some mouthwashes. In this study Eucalyptus camaldulensis leaves extracted with water and ethanol 96°and the antimicrobial effects of extracts were evaluated by "using the method of Collins" and "disk agar diffusion method". Antimicrobial properties of Carboxy Methyl Cellulose [CMC] films containing 20, 40, 60, and 80 mg/ml concentration of the extract studied against on Streptococcus pyogenes PTCC 1447, Pseudomonas aeruginosa PTCC 1310 and Staphylococcus epidermidis PTCC 1435. The results showed that aqueous and alcoholic extract were quite effective in 2000 ?g/ml concentration on Streptococcus pyogenes and Staphylococcus epidermidis and have inhibition effect, while both extracts have no certain antimicrobial effect on Pseudomonas aeruginosa. Minimum Inhibitory Concentration [MIC] of ethanolic extract of Eucalyptus camaldulensis leaves were performed for each microorganism. Minimal Bactericidal Concentration [MBC] for bacteria was performed using the dilution method. The edible films containing mangrove extract presented more effective impact on the growth of Streptococcus pyogenes than Pseudomonas aeruginosa [p<0.05]. The result indicates extracts of Eucalyptus camaldulensis leaves have the greatest effect on gram-positive bacterium Streptococcus pyogenes. As a result, aqueous and ethanloic extracts of Eucalyptus camaldulensis leaves, have been strong antimicrobial activity against many food pathogen bacteria

Investigating the minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC] of the Lavandula stoechas L. and Rosmarinus officinalis L. extracts on pathogen bacterias "in vitro"

The infections risk related to pathogenic germs increases at the present time considering the increased resistance which certain microbes acquire, whose usual antibiotics are ineffective to treat the infectious disease. The aim of this study was to determine antimicrobial effect of the aqueous and ethanolic extracts of Lavandula stoechas L. and Rosmarinus officinalis L. on Listeria monocytogenes PTCC 1297 ¡Bacillus cereus PTCC 1154¡ Enterobacter aerogenes PTCC 1221¡ Enterococcus faecalis PTCC 1237 and Salmonella typhi PTCC 1609 "in vitro". In this experimental study, after collecting plants from of Razavi Khorasan province, the extraction was carried out by the maceration method, after antimicrobial effect of the extracts evaluated by two methods, "Collins method" [spreading of the extract on medium surface] and "disk agar diffusion method". The Minimum Inhibitory Concentration [MIC] and Minimum Bactericidal Concentration [MBC] for both species determined by using a dilution method. Statistical analysis was carried out by analysis of variance [ANOVA].The results show that aqueous and ethanolic Lavandula stoechas L. extracts were quite effective in 2000 micro g/ml concentration on Listeria monocytogenes ¡Bacillus cereus and Enterococcus faecalis. The results indicate that ethanolic extracts of Lavandula stoechas L. have the greatest effect on gram-positive bacterium. The result shows that MIC of Lavandula stoechas L. leaves of the aqueous and ethanolic extracts for Enterobacter aerogenes was 32 and 16 mg/ml respectively. The result shows that MIC of Rosmarinus officinalis L. leaves of the aqueous and ethanolic extracts for Enterobacter aerogenes was 128 and 64 mg/ml respectively.The Lavandula stoechas L. and Rosmarinus officinalis L. extracts presented the more effective impact on the growth of gram-positive bacteria than gram-negative bacteria [p<0.05]

Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria "in vitro"

The aim of this paper was to investigate the antibacterial potential of Kelussia odoratissima Mozff extract against Gram-negative and Gram-positive bacteria. Karafs-eKoohi with the scientific name of Kelussia odoratissima is an Iranian endemic edible plant in the middle region of Iran with enormous use as food, spice and medicinal herb. The antibacterial effect of the extracts was investigated using pour plate and disk diffusion methods. Minimum Inhibitory Concentration [MIC] and Minimum Bactericidal Concentration [MBC] were also studied using the dilution method. Repeated measure ANOVA was used for data analysis. The results showed that in disk diffusion method all concentrations of ethanolic extract had inhibitory effect against Bacillus subtilis and Staphylococcus aureus. Minimum Inhibitory Concentration [MIC] of Kelussia odoratissima leaves of aqueous and ethanolic extracts for Bacillus subtilis and Staphylococcus aureus were 16 and 8 mg/ml, and for Enterobacter aerogenes were 32 and 16 mg/ml, respectively. Minimum Bactericidal Concentration [MBC] of Kelussia odoratissima leaves of aqueous and ethanolic extracts for Bacillus subtilis and Staphylococcus aureus were 32 and 16 mg/ml, and for Enterobacter aerogenes were 64 and 32mg/ml, respectively. The results showed that the extract of Kelussia odoratissima had a satisfactory antimicrobial activity and the ethanolic extract of Kelussia odoratissima leaves had greater inhibitory effects on the strains studied compared to aqueous extract in vitro. A significant correlation was also observed between zone of inhibition and concentration of extracts

[Isolation, biochemical and molecular identification of probiotic bacteria from traditional dairy products of Sabzevar]

Background: Probiotics are beneficial and non-pathogenic microorganisms. Isolation of probiotic bacteria from traditional dairy products can not only lead to the isolation of probiotic bacteria with special characteristics, but it can offer a good approach for the mass production of traditional dairy products containing natural probiotic bacteria.

Materials and Methods: After collection of dairy products samples from different regions of Sabzevar, they were continuously cultured on the specific media of MRS and MRS Broth. Initial identification of isolates was performed by gram stain, motility test, nitrate reduction test, growth at 15 and 45 °C, growth at pH: 9/6 and fermentation capability of 11 different sugars. To identify desired strains more precisely, the 16S rDNA gene was amplified by PCR with specific primers and then sequenced and BLASTed. Acidic and bile salts conditions tolerance tests were performed for the final confirmation of desired strains.

Results: After continuous culture on specific agar media, 16 strains for further analysis were selected. In early identification of isolates by phenotypic methods, 14 strains were positive. To identify these strains more precisely, the 16S rDNA gene was amplified. Following molecular identification and sequencing of 16S rDNA gene, the BLAST sequence similarities were found with Lactobacillus planetarium. In addition, acidic and bile salts conditions tolerance tests showed that these bacteria had the best growth pattern at PH: 4 and they were able to grow in the presence of bile salts.

Conclusion: Biochemical results showed that the most common strains in the tested dairy products are Lactobacillus. These results also confirmed by the molecular tests. Acidic and bile salts conditions tolerance test, as a main characteristic of probiotic bacteria, showed that the strain was able to withstand these conditions.

Comparison of the results of orbital floor reconstruction using different materials

Complications of orbital floor reconstruction is a big challenge for patients and specialists. The purpose of this study was to investigate the results and complications of the orbital floor reconstruction using different materials and to compare them. In This Retrospective study consisted of 12 cases with orbital floor fractures treated at Imam Reza Hospital of Tabriz Medical University, Iran, between June 2012 and May 2013. Patients' clinical data were retrospectively analyzed for gender, age, reconstruction material, and complications. Autogenous bone, Nasal septum, titanium mesh, and Medpor were implanted under the periosteum of the orbital floor in 3,2,4 and 3 cases respectively. All of the patients except 3 cases, showed satisfactory appearance. Diplopia and limitation of globe movement were resolved in 50% of the cases. Complete infraorbital nerve recovery were observed in 2 cases after 8 weeks. Subciliary incision was adopted in our surgery. There was no statistically significant difference among groups of patients in regard to the effectiveness of treatment using different materials. Appropriate diagnosis, early intervention, proper material selection and accurate orbital floor reconstruction were the key factors for successful treatment

Effect of aqueous and ethanolic extract of Eucalyptus camaldulensis L.

Herbs have been used for its medicinal properties from several thousand years ago. Herbs, essences and extracts, possess a variety level of biological activities and antimicrobial activities of a large number of them have been proved. Eucalyptus is one of these plants which the anti-virus effects of its extract has long been used to treat influenza and colds in most parts of the world. The aim of this study is evaluating antimicrobial effects of aqueous and alcoholic Eucalyptus camaldulensis leaves against some of the important food pathogens. Antimicrobial effects of extracts were evaluated on Staphylococcus aureus PTCC 2592, Escherichia coli PTCC1330 and Penicillium digitatum by "using the method of Collins" and "disk agar diffusion method". The results showed that aqueous and ethanolic extract were quite effective in 2000 micro g/ml concentration on Staphylococcus aureus and Penicillium digitatum, while both extracts have no certain antimicrobial effect on Escherichia coli. In "disk agar diffusion method" 20, 40, 60 and 80 mg/ml aqueous and ethanolic extract concentrations, was inhibition effect on Staphylococcus aureus and Penicillium digitatum, and 60 and 80 mg/ml aqueous and ethanolic extract concentrations, has deterrent effect on Escherichia coli, but at 20 and 40 mg/ml concentrations, no inhibitory effect on Escherichia coli was observed. Minimum Inhibitory Cconcentration [MIC] of ethanolic extract of Eucalyptus camaldulensis leaves and Minimal Bactericidal Concentration [MBC] for bacteria and Minimum Fungicidal Concentration [MFC] for fungi were performed. The results indicate that ethanolic and aqueous extracts of Eucalyptus camaldulensis leaves have the greatest effect on gram-positive bacteria Staphylococcus aureus. Escherichia coli were resistant to most of the aqueous and ethanolic Eucalyptus camaldulensis extracts. In conclusion, using Eucalyptus as a natural antimicrobial compounds in vitro have considerable antimicrobial ability over the studied strains

Intermittent versus continuous phototherapy for reducing neonatal hyperbilirubinemia

Clinical studies comparing intermittent versus continuous phototherapy for reducing neonatal hyperbilirubinemia have produced conflicting results. This study was conducted to compare the efficacy of intermittent with continuous phototherapy. This study was performed on 114 neonates with indirect hyperbilirubinemia. Inclusion criteria were body weight above 2000 grams, absence of other concomitant diseases, and hyperbilirubinemia neither requiring intensive phototherapy nor exceeding the range of exchange transfusion. The neonates were randomly divided into two groups. Continuous phototherapy group received phototherapy on and off for 2 hours and half an hour respectively and the intermittent phototherapy group on and off for one hour. The phototherapy units were identical and serum total bilirubin levels were measured every 12 hours after starting phototherapy. Two groups were matched regarding weight and risk factors such as ABO and Rh incompatibility. The difference of total serum bilirubin levels between two groups was insignificant at the start of phototherapy and also after 12, 24, 36 and 48 hours [P > 0.2]. Intermittent phototherapy defined as one hour on and one hour off is as effective as continuous phototherapy defined as 2 hours on and half an hour off, in reducing total serum bilirubin level in full term babies