Clinical evaluation of the feasibility of interstitial Nd: YaG Laser in the treatment of gingival overgrowth

Nd:YAG laser have been recently utilized as a new modality for excision of exophytic lesions with minimal post operative sequlae. Thus, the present investigation was carried out to evaluate the use of Nd: YAG laser in the treatment of two different types of gingival enlargement, particularly those with history of recurrence. 10 cases with pyogenic granuloma [PG] and 6 cases with peripheral giant cell granuloma [PGCG] were treated by Nd: YAG laser with average power 3- 5W, pulse duration one second delivered interstitially by a fiberoptic with gauge 400 micro m. The result showed complete resolution of all cases and no recurrence was detected up till one year postoperative. Consequently Nd: YAG laser application seems to be a successful treatment modality for gingival enlargement

5-years experience of laser treatment of pyogenic granuloma of head and neck

Twenty-three cases of pyogenic granulomas [PG] were treated in the National Institute of Laser Enhanced Sciences [NILES], Cairo University in the period between 2000-2004. Different types of lasers were used to treat such lesions, which were located either on the skin or the mucosa of head and neck. Eleven patients were males and twelve patients were females. Four of them were pregnant. The age ranged between 12 and 71 years with an average of 38.3 years. All cases were cured after a single laser session, except three cases needed multiple laser sessions. Laser treatment, under local anesthesia as an office-based procedure, proved to be an effective method to remove different pyogenic granulomas affecting skin and mucosa of head and neck without any considerable complications and with minimal postoperative pain

Assessment of anti-inflammatory effect of 830nm laser light in acute myofascial pain of temporomandibular joint origin using c-reactive protein and erythrocyte sedimentation rate levels

This study was a randomized blind study performed on twenty adult patients with acute myo-lascial pain of ternporomandibular joint origin. The patients were randomly and equally divided into two treatment groups. In addition to the standard antiinflammatory regimen received in both groups, Group A patients! mean age 33.6 years received laser therapy in the form of 830 nm soft laser of 80 mWatt power maximum output in contact mode in the joint articulation and musculature three times a week for a total period of three weeks [nine sessions]. Group B patients [mean age 28.4 years] received sham laser [0 m1/cm2] Clinical parameters as maximal interincisal opening, lateral movements of the jaw, number of tender points, visual(sensual) analogue scale as well as hae-matological indices such as C-reactive protein and erythrocyte sedimentation rate were all measured and recorded to assess treatment over a period of three weeks. This study has demonstrated a statistically significant superiority [ p < 0.05]of results and improvement of symptoms in patients re-cemrvg, compared were restricted to values did not differ significantly and showed a more symmetrical distribution for both groups. It can therefore be deduced, based on the results demonstrated in this study that LILT in acute myofascial pain of TMJ origin is a successful treatment modality and that while CRP values could be extremely useful in ascertaining the diagnosis of an acute case, ESR can be much more useful in monitoring the response to treatment

Evaluation of interventional Nd : Yag laser surgery treatment of soft-tissue hemangiomas and vascular malformations of the oral cavity: a clinical study

The treatment of vascular malformations remains controversial because the oral and max-illofacial surgeon must always balance potential morbidity with projected benefit. A minimally in-vasive technique is described, with the neodymium: yttrium-aluminium-garnet [Nd:YAG] laser being used for interstitial photocoagulation of hemangiomas and vascular malformations in a series of twelve patients of mean age 12.4 years. Interstitial photocoagulation was performed by using the fiber-based Nd:YAG laser. This was accomplished by introducing the laser fiber with an angiocath and delivering various amounts of energy [6VV to 10W]. There were no complications. The procedures were easy to perform and well tolerated, with all patients being discharged on the same day of surgery. The following outcome measures were statistically analyzed: Decrease in the area of the target lesion, [% reduction] and number of treatments required to achieve reduction in size and their relation to size of the lesion. A follow up period of 6 months showed no regression in all but 2 patients with good cosmetic results. The range of reduction in size was 100% for hemangiomas while it ranged from 71.6%[2 weeks postoperative] to 96.2% at 6 months for capillary malformations. Capillary malformations needed greater number of sessions to be fully treated with a highly sig-jjficant p value [p< 0.00]]. The percent reduction in size was significantly different in capillary malformations than for hemangiomas at 3rd day, 2nd week, till the end of the follow up period. Correlation between the number of sessions needed and the size of the lesion showed a relatively high coefficient of positive correlation [r =.67]. Interstitial Nd:YAG laser photocoagulation offers the oral surgeon a therapeutic alternative in the treatment of refractory vascular malformations or in cases where there is a considerable projected surgical morbidity. Although multiple treatments may be required, the procedure is effective, simple and well tolerated

Tumor eradication using methylene blue induced photodynamic therapy in mouse model: an experimental study

Thirty nude female Swiss Albino mice of weight 18-22gm were used in this study. The mice were subdivided into three subgroups of 10 mice each. Group A was to be maintained without any tumor cell implantation to provide normal cell readings on the spectrometer and to supply a reference for normal histopathological specimens. Groups B and C were to be injected with tumor cells and methylene blue dye intralesionally. Group C mice were to receive PDT treatment while Group B mice were intended to act as a control for such treatment modality by receiving MB+ injection only without any irradiation. Measurements performed included the change in tumor volume as compared to the value recorded at the start of the experiment, after a one week incubation period. Ceased mice were dissected to remove the tumor mass and thereby measuring its volume. Survival rates for each group were calculated throughout the following weeks. The tumor investigated in this study was a solid Ehrlich carcinoma which is a transplantable, poorly differentiated malignant tumor which appeared originally as a spontaneous breast carcinoma in mice. A line of Ehrlich ascites carcinoma [EAC] was supplied from the breading unit of the National Cancer Institute Cairo University. A line of 1X10 [EAC] cells was transplanted by subcutaneous inoculation in the submandibular area. The tumor developed seven days after injection at which time its size was considered appropriate for PDT treatment. Thereafter, Methylene blue 0.1 ML concentration 2% was injected into the lesion. The statistical significance of the therapeutic effect of PDT as a treatment modality was analyzed. Methylene blue intratumoral injection alone without irradiation did not result in any macroscopic cytotoxicity or inhibition of tumor growth. PDT as a treatment ' modality proved to be highly effective. Irradiation of the incubated tumors with 550 J/cm[2] led to almost complete macroscopic regression of the tumors towards the end of the third week. Histological examination of the scar proved complete destruction of the lesion. Overall comparison of tumor volume regardless of the time of death or sacrifaction showed a highly statistical significant difference in favor of PDT treated group. [p=0.001] Median overall survival was 4 weeks in PDT treated group compared to one week in the group injected with methylene blue only, [p = 0.02]. The variation in the structural integrity of the cells as studied by Raman spectroscopy indicated a] shifting of bands which indicate chemical reaction and structural changes, b] difference in relative intensities of the bands of untreated and treated specimens. Readings were recorded for Group A [normal cells] mice, Group B [methylene blue injected only] mice and Group C [PDT] treated mice. The results of the Raman spectroscopy of the test specimens were recorded. The spectra of transformed malignant tissues differed markedly from those of normal and treated tissues .In most regions of spectra, the difference was evident as increase in the intensity of the absorbance from malignant tissues in comparison to normal and treated tissues. It thus seems that Raman spectroscopy could be used for the study and characterization of cells at various stages of their life cycles. Our results support the feasibility of developing spectroscopy as an easy yet sophisticated method for detecting malignant cells and possibly also for discriminating among cells at various stages in their life cycles