RESUMO
There are many reports regarding to effects of Granulocyte colony-stimulating factor [G-CSF] and stem cell factor [SCF]alone in liver repair .But conflicting data have been reported regarding the role of growth factors such as G-CSF and SCF in the liver regeneration system. Also, there is not such data regarding to effects of co-administration both of G-CSF and SCF in the liver damage condition. An experimental model of rat liver damage induced by the thioacetamide. Five different groups of animals receiving 0.9% NaCl, TAA alone, TAA + G-CSF, TAA + SCF and TAA + [G-CSF+SCF].The activity of glutamate pyruvate transaminase [GPT/AlT]and glutamate oxaloacetate transaminase [GOT/AST] were measured after the thioacetamide [TAA] injection and the administration of combination of G-CSF +SCF for 12 weeks. Also histological tests were carried out at the end experiments. The pre-treatment of combination of G-CSF and SCF for 12 weeks reduced the degree of liver injury. The mean of GOT activity was 61.24 [U/L] in the G-CSF +SCF and versus 132.86 in the TAA-alone group. These differences in the GOT activity were statistically significant [P<0.05]. Also, in the G-CSF +SCF and TAA group the mean of GPT activity [4.35 versus 11.79, respectively] were lower than in the TAA-alone group, this difference was statistically significant [P<0.05]. Liver sections from a rat treated only with TAA, showing damage, but TAA and G-CSF + SCF no significant damage is present. On the other hand histological results revealed a very mild degree of inflammation were observed in the livers of the combination of G-SCF+SCF and TAA-treated rats compared to TAA only treated group. Biochemical and microscopic analysis revealed that combination of G-CSF and SCF pre-treatment significantly enhances liver regeneration after TAA-induced liver injury