Prooxidant-antioxidant balance and malondialdehyde over time in adult rats after tubal sterilization and vasectomy / 대한생식의학회지

OBJECTIVE: Sterilization (tubal sterilization and vasectomy) is a widely applied contraceptive method worldwide. Although most studies have described sterilization as a safe method, there are reports of tubal ligation (TL) and vasectomy complications. The aim of this study was to evaluate the effects of TL and vasectomy on the serum oxidative stress, specifically prooxidant-antioxidant balance (PAB) and malondialdehyde (MDA) levels, over time. METHODS: Male and female rats were classified into vasectomy, sham-vasectomy, TL, and sham-TL groups, respectively. The PAB and MDA levels were measured on days 15 and 45 and months 3 and 6 after the intervention. For female rats, blood sampling was performed during the diestrous phase and estradiol and progesterone were also measured. RESULTS: Serum PAB and MDA increased after TL (p<0.05). Vasectomy increased serum MDA remarkably after 45 days, 3 months, and 6 months (p<0.05). After vasectomy, serum PAB also increased although not significantly. Serum estradiol and progesterone decreased remarkably in the TL group compared to the sham group (p<0.05). CONCLUSION: Bilateral TL and vasectomy both increase the serum oxidative stress; however the imbalance after TL was very noticeable. As for the TL, the reduction of serum estrogen levels can be involved in this imbalance. Complications followed by TL or vasectomy could be due to increased levels of oxidants. Thus, prescribing antioxidants during and or after surgery may be a solution.

Prevalence of human T-Lymphotrophic Virus [HTLV] 1,2 Among tissue donors in Iranian tissue bank

Iranian Tissue Bank prepares a wide range of human tissue homografts such as; Heart valve, Bone, Skin, Amniotic membrane and other tissues for different clinical applications.The purpose of this study was to determine the prevalence of HTLV in tissue donors from 2001 to 2006 in Iranian Tissue Bank/ Tehran University of Medical Sciences. 1548 tissue donors were studied during a 5-year period by ELISA assays. HTLV1,2 - antibodies were tested on all donors along with other tests upon American Association of Tissue Banks [AATB] standards. 25 [1.61%] out of 1548 tissue donors were HTLV positive. 17 donors were male and 8 donors were female. Female to male ratio was approximately 47%. Regarding the prevalence of HTLV among tissue donors and importance of cell and tissue safety and quality assurance, we recommend that all cell and tissue banks should be involved with serological and other complementary tests such as PCR [Polymerase Chain Reaction] for HTLV