Antimicrobial Effect of Essential Oil of Thymus capitatus from Northern Cyprus and Its Gargle Preformulation

Aims:Theaim of this study is to prepare herbal gargle preformulations making use of essential oil of aerial parts of Thymus capitatusgrowing wild in Northern Cyprus and comparing antimicrobial efficacy between these formulationswith pure essential oil.Place and Duration of Study: Department of Pharmaceutical Technology, Faculty of Pharmacy, Near East University, Nicosia, TRNC and Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ankara University, Ankara, Turkey, between January 2017 and June 2017.Methodology: Preformulations with three different concentrations of Thymus capitatusessential oil were prepared by using simple preparation method.Preformulation studies were done in lab with less than 500ml. There is not specific process parameters. Manufacturing process and process parameters will be clarified with further studies.These compositions and the essential oil were tested in-vitrofor antimicrobial activity studies by using broth microdilution method. Staphylococcus aureusATCC 43300, Staphylococcus aureusATCC 25923, Staphylococcus epidermidisATCC 12228, Bacillus subtilisATCC 6633, Escherichia coliATCC 25922, Enterococcus faecalisATCC 29212, KlebsiellapneumoniaeRSKK 574, Salmonella paratyphiC, Pseudomonas aeruginosaATCC 9027, Pseudomonas aeruginosaATCC 27853 as bacteria and Candida albicansATCC 10231, Candida parapsilosisATCC 22019 as yeasts were used for antimicrobial activity tests. Results: All trialswere found to be more effective than EO, and a significant effect was observed when compared to the values of standard antimicrobial agents.Conclusion:TheThymus capitatusgrowing in Northern Cyprus could be used as a herbal raw material, essential oil source in developing herbal gargle preformulationsto reduce fungal and bacterialinfections in mouth

In vitro Antimicrobial Activity of Various Mouth Rinses against Streptococcus mutans, Lactobacillus casei, L. acidophilus and Candida albicans.

Aim: The aim of this in vitro study was to determine the antimicrobial activities of commonly used mouth rinses against different oral microorganisms. Methodology: Six commercially available mouth rinses (Colgate Total, Colgate Plax, Colgate Pro-argin, Oral-B Pro-expert, Listerine and Oderol) were used in this study. The antimicrobial activities of mouth rinses against Streptococcus mutans ATCC 25175, Lactobacillus casei RSKK 900, L. acidophilus ATCC 11975 and Candida albicans ATCC 10231 were evaluated by disc diffusion and minimum inhibitory concentration tests. Klorhex® was used as positive control. Experiments were repeated twice. Results: Colgate Total, Colgate Plax, Oral B Pro-expert and Oderol showed good antimicrobial activity against test microorganisms in comparison with Colgate Pro-argin and Listerine. Conclusion: Chlorhexidine and cetylpyridinium chloride containing mouth rinses were found more active than other products tested. Main motive of mouth rinses is removing the microorganisms from the mouth although their effectiveness depends on the formulation, concentration, quantity of ingredients and duration of rinsing.