RESUMO
Background: the impairment of wound healing in the diabetic patients is an important clinical problem affecting millions of patients worldwide. This significant health care problem still lacks effective therapy. The ideal wound dressing is resistant to external forces and pathogens. It reduces patient discomfort and achieves good cosmetic results
Aim of the work: this study aimed to compare the effect of silver nanoparticles versus silver sulfadiazine on diabetic wound healing in the adult albino rat
Material and Methods: twenty adult female albino rats were included in this study. The animals were categorized into four groups, 5 rats each: group I [control].Group II [untreated diabetic wound]: diabetic rats that left untreated for 15 days of wound excision. GroupIII: diabetic rats in which silver sulfadiazine cream was applied on the wound daily for 15 days after thin skin excision. GroupIV: diabetic rats in which nanosilver-ointment was applied on the wound daily for 15 days after thin skin excision.At the end of the experiment, the specimens were taken and processed for histological and immunohistochemical studies. Morphometric and statistical studies were carried out
Results: examination of thin skin sections of nanosilver treated group revealed continuous epidermis with differentiated keratinocytes with proliferating basal layer .The dermis showed increased collagen deposition ,decreased p53 and increased VEGF expression compared to SSD treated group
Conclusion: silver nanoparticles could be more effective than silver sulfadiazine in treatment of the diabetic wound
RESUMO
Twenty-five adult male albino rats weighing 150-200 gm were used in this study. The animals were divided into five groups of five animals each. The results after intake of the therapeutic dose revealed fusion of the minor feet processes of the podocytes and apparent increase in the thickness of the glomerular basement membrane in comparison with the control group. Both proximal and distal convoluted tubules showed disappearance of their basal infoldings and evident mitochondrial degeneration in the form of swelling and loss of their cristae and dilated cisternae of rER were also noticed. In addition, microvilli of proximal convoluted tubules were lost in focal areas. The same changes, but in more severe form, were noticed after the intake of the toxic dose. In addition, there were tubular nuclear changes in the form of irregularity of contour, pyknosis or decreased density of chromatin