The study of the intestinal microbiota among Mongolian Adults, it related some factors / Монголын Анагаах Ухаан

Background@#The intestinal microbiota of Mongolians and its composition is of great interest of researchers, a few studies have did in this fields. Maybe Mongolian encompass a uniquely wide range of environmental conditions, ethno geographical cohorts and traditional nomadic lifestyles.@*Goal@#We aimed to determine the amount of gut microbiota, including Lactobacillus and Bifidobacterium in the fecal samples of relative healthy Mongolian adults residing in various regions of Mongolia by conventional culture method and PCR. @*Material and Methods@#The study was performed population based cross sectional study in healthy volunteers. In this study, 256 relative healthy Mongolian adults with no history of gastrointestinal associated diseases were enrolled between July 2018 and April 2019. Each participants was asked to complete a questionnaire containing 164 questions about demographics, physical activity, dietary habits. Fecal samples were collected for Lactobacillus and Bifidobacterium analysis using culture method and determination of genus of Bifidobacterium sрp and Lactobacillus spp by PCR. ResultsParticipants had a mean age of 38.9±12.8 years. The mean values of Lactobacillus by culture method were 5.9±1.28 and 6.24±0.94 log10 CFU/ml (4.67х106 , 4.66х106 CFU/ml), respectively. The abundance of Lactobacillus had a positive correlation with grams for fiber and amount of bifidobacterium ((r= 0.495, р<0.001, r=0.288, p<0.05), respectively). Significant difference were observed between groups of milk frequency per day for amounts of lactobacillus. In adult intestinal tracts, B.Bifidum was the most common taxon 31 (29%) followed by B. angulatum 14 (13.1%), B. adolescentis 10 (9.3%), B. catenulatum group 10 (9.3%), B. longum 9 (8.4%). B. lactis, B. breve, B. dentium and B. gallicum were subdominant species. @*Conclusion: @#The mean amount of Bifidobacterium and Lactobacillus of all participants were 6.24±0.94 and 5.9±1.28 log10 CFU/ml (4.66*106 , 4.67*106 CFU/ml) respectively. The Lactobacillus abundance of healthy adults was higher in region of Khangai, East and West of Mongolian than other regions. The composition of lactobacillus altered with ageing. Significant correlations were found between fiber, fats, potato and amount of Lactobacillus. Keywords: Bifidobacterium, Colony forming unit, Gut microbiota, Lactobacillus

Detection of chromosome 13 (13q14) deletion among Sudanese patients with multiple myeloma using a molecular genetics fluorescent in situ hybridization technique (FISH)

Multiple myeloma (MM) is a neoplastic plasma cell dyscrasia with an incidence of 4-4.5 per 100,000 population per year. It is regarded as the second most prevalent blood cancer (10%) after non-Hodgkin lymphoma. The objective of this study was to investigate the mutational change in chromosome 13 (13q14) among Sudanese MM patients and to identify the association between extent of plasma cell infiltration in the bone marrow, albumin level and deletion of 13q14 by an analytical case control study. Materials and Methods: 15 patients were enrolled in the study. 11 bone marrow samples were collected from MM patients at different stages of the disease and 4 samples were from patients with conditions other than MM as control. Plasma cells were counted from bone marrow smears and fluorescence-in-situ hybridization (FISH) was performed using Fluorophore labeled DLEU1 (13q14) LSI (local specific identifier) probe designed as a dual-colour assay to detect deletion at 13q14. Heparanized sample was taken for estimation of serum albumin in all patients. Results: 13q14 deletion was detected in 6 (54.5%) MM patients while one (9.1%) patient showed monosomy. All relapsed MM (27.3%) had 13q14 deletion. Surprisingly almost all patients studied had normal albumin level. The study could not show whether the deletion is implicated in the pathogenesis of multiple myeloma.

Dental considerations in pregnancy: review / Considerações odontológicas na gravidez: revisão

OBJETIVO: Este artigo propõem-se a revisar as alterações ocorridas na mulher durante a gravidez. Os cirurgiõesdentistas devem conhecer os procedimentos seguros e os que devem ser evitados durante as várias fases da gravidez. DISCUSSÃO E CONCLUSÃO: Este artigo aborda as várias alterações sistêmicas vistas na mulher grávida e como essas alterações devem ser manejadas na execução de procedimentos odontológicos. Os cirurgiões-dentistas devem ter sólido conhecimento a respeito dos efeitos da radiação sobre o feto e também dos efeitos de várias drogas usadas durante o tratamento odontológico. Com comunicação adequada e bom conhecimento, várias complicações podem ser evitadas durante a gravidez, culminando em benefícios para a mãe e o bebê.

OBJECTIVE: This article is aimed to review the changes occurring in a woman during pregnancy. Dentists should know what procedures are safe and those to be avoided during various phases of pregnancy. DISCUSSION AND CONCLUSION: This article reflects the various systemic and local changes seen in a pregnant woman and how these changes should be considered while carrying out dental procedures. Dentists should have a sound knowledge about effects of radiation on the fetus and also effects of various drugs used during dental treatment. With proper communication and good knowledge, various complications can be avoided during pregnancy and would be beneficial to both the mother and the fetus.

Tooth fragment reattachment after late retrieval (three months) from the lower lip: case report / Colagem de fragmento dentário recuperado tardiamente (após três meses) do interior do lábio inferior: relato de caso

OBJECTIVE: To present and discuss a case of child who sustained a complicated crown fracture,with the lost portion of tooth embedded in his lower lip. RESULTS: Tooth fragment was surgicallyremoved and successfully reattached to the tooth using acid etch and dentin bonding resin technique.

OBJETIVO: Apresentar e discutir um caso de criança que sofreu fratura complicada de coroadentária, com parte do dente perdida e alojada no lábio inferior. RESULTADOS: O fragmentodentário foi cirurgicamente removido do lábio e colado, com sucesso, ao dente, utilizando-seataque ácido e cimentação com compósito.

Oral health status in geriatric diabetics / Condições de saúde bucal em pacientes diabéticos geriátricos

OBJECTIVE: To determine if any difference exists in the oral health status between geriatric patients with diabetes mellitus and the normal population. SUBJECTS AND METHODS: The oral health status of 100 metabolically controlled geriatric diabetic patients attending the Diabetes Clinic of the Manipal Hospital, were prospectively assessed over six months and compared with that of 50 non-diabetic acting as controls. RESULTS: The mean duration of diabetes was 100.5±85.1 months. The degree of hyposalivation between the two groups was statistically significant (p < 0.05). No significant difference was observed in the taste, burning mouth sensation, angular cheilitis, glossitis, and stomatitis status of the two groups and no pathgnomonic lesions or alterations could be observed in relation to the disease. CONCLUSION: It was concluded that, with adequate metabolic control, the oral health status of a diabetic may not be significantly different from that of a non-diabetic except for xerostomia.

OBJETIVO: Avaliar possíveis diferenças na saúde bucal de pacientes geriátricos com diabetes mellitus em comparação a uma população normal. CASUÍSTICA E MÉTODO: A condição de saúde bucal de 100 pacientes geriátricos diabéticos, metabolicamente controlados, atendidos na Clínica de Diabetes do Hospital Manipal foram avaliados prospectivamente durante seis meses e comparados com 50 pacientes geriátricos não diabéticos (grupo controle). RESULTADOS: A duração média do diabetes foi 100 m5 +- 85,1 meses. O grau de hiposalivação entre os dois grupos foi estatisticamente significante (p < 0,05). Não foram encontradas diferenças significativas no paladar, sensação de queimação bucal, queilite angular, glossite e estomatites entre os dois grupos. Não foram observadas lesões patognômicas ou alterações relacionadas ao diabetes. CONCLUSÃO: Concluiu-se que, com controle metabólico adequado, as condições bucais de pacientes diabéticos geriátricos pode não ser significativamente diferente das de um não diabético, com exceção da xerostomia.

Surgical management of nasopalatine duct cyst: case report / Tratamento cirúrgico do cisto nasopalatino: relato de caso

OBJECTIVE: To present a case and discuss the clinicopathological characteristics of nasopalatine duct cyst and discuss the etiology, diagnosis, treatment and prognosis, with a review of the literature. DISCUSSION: Nasopalatine duct cyst occurs in approximately 1% of the population. Presentation may be asymptomatic or include swelling, pain, and drainage from the hard palate. Surgical treatment was carried out under local anesthesia and comprised the dissection and removal of the cyst adopting a usually palatine approach.

OBJETIVO: Apresentar um caso e discutir as características clínico-patológicas do cisto de ducto nasopalatino, discutinfo a etiologia, diagnóstico, tratamento e prognóstico, revisando a literatura. DISCUSSÃO: Os cistos naso-palatinos ocorrem em aproximadamente 1% da população. A apresentação pode ser assintomática ou incluir edema, dor e drenagem purulenta do palato duro. O tratamento cirúrgico foi sob anestesia local e constituiu de dissecção e remoção do cisto, via acesso palatino.

interdisciplinary approach for restoring function and esthetics in a patient with amelogenesis imperfecta: a case report

Amelogenesis Imperfecta has been defined as a group of hereditary enamel defects not associated with evidence of systemic disease. Restoration for patients with this condition should be oriented toward the functional and aesthetic rehabilitation. The importance of treating the Amelogenesis Imperfecta patient is not only important from a functional standpoint, but also from a psychosocial health standpoint. The complexity of the management of patients with Amelogenesis Imperfecta requires careful considerations of patient expectations for a successful outcome of the treatment. The purpose of this case report is to present the aesthetic and functional rehabilitation of the teeth with an overall enhancement of personality of a 24-year-old patient with Amelogenesis Imperfect

Management of a patient with congenitally missing lateral incisor: a multidisciplinary team approach / Tratamento de um paciente com ausência congênita de um incisivo lateral: uma abordagem multidisciplinar

OBJECTIVE: The present paper highlights a case where the patients presented with spacing in the upper anterior region due to missing right lateral and peg shaped left lateral incisor. METHOD AND RESULTS: The initial treatment was standard edgewise fixed orthodontic appliance to distalize the canine, correct the bite and create space for the lateral incisors and correct the midline. CONCLUSION: After the active phase of fixed orthodontic treatment, an implant was placed and the peg shaped lateral incisor was built up with composite.

OBJETIVOS: O presente artigo refere-se a um caso em que o paciente apresentava espaços na região anterior da maxila devido à ausência de incisivo lateral direito e incisivo lateral esquerdo com coroa deformada. MÉTODO E RESULTADO: O tratamento inicial foi ortodontia fixa (edgewise) para distalizar o canino, corrigir a mordida e criar espaço para o incisivo lateral e corrigir a linha média. CONCLUSÃO: Após a fase ativa da ortodontia, utilizou-se um implante adequado na região do lateral direito e reconstruiu-se a coroa deformada do incisivo lateral esquerdo com compósito.

Comparison of Helicobacter pylori Virulence Factors, Intcrleukin-1 Polymorphisms ni Patients and Endoscopic and Histological I-indiums of Stomach and Duodenum / Шинэ санаа Шинэ нээлт

BACKGROUND The aim of the study is to detect and define the role of H. pylori virulence factors and host IL-1 polymorphisms to prevent from further gastric cancer. mwom 5ml of blood samples were collected from each of 42 patients who had abdominal complaint, after informed consent was obtained. All patients were Mongolian nationality. The biopsy specimens were stored in liquid nitrogen and homogenized before DNA isolation. After tissue lysation with proteinase K. DNA isolation was performed with "Promege" tissue kit. according to the manufacturer's instructions. PCR amplification of H. pylori gene loci was performed for the cagA gene and the vacAs mosaics vac As 1 and vacAs2. RESULTS Result of histological findings shows 84.7% from all patients were diagnosed with II. pylori infection 83% (35/42). Histologically LI'G 50% (42/21). Gastric atrophy 30% (42/13). Intestinal metaplasia 9% (42/4). Gastroduodcnal ulcer 4% (42/2), Dys¬plasia 11% (42/5), Adinocarcinoma 2% (42/1), 3 patients (42/3, 7%) were none patho¬logic change. 62% (26/42) patients infected with H. pylori, as determined by Urease test. H. pylori were investigated in all 42 patients and 83% (35/42) were infected with II. pylori, as determined by histology (haematoxylin- eosin and (iiemsa-stained). Strain characteristics of H. pylori were investigated in all 42 patients and 83% (35/42) were infected with //. pylori, as determined by UreC PCR. Result of histological findings revealed Bacilla form 48,5% (17/35), Coccoid form 28,5% (12/35), mixed form 14% (5/35) from all patients were found //. pylori. 76% (13/17) of all patients were revealed coccoid form of H. pylori were taken anti-//. pylori treatment. The vacAs 1 genotype was found in 38% (16/42) of all UreC+ patients, and cagA was found in 23% (10/42) of UreC+ patients. 16.9% of all patients were IL-RN*2 positive (7/42), (IL-1B 31C/51 IT).

Non-syndromic multiple odontogenic keratocysts: report of case / Keratocistos múltiplos não-sindrômicos: relato de caso

Odontogenic keratocysts (OKCs) are epithelial developmental cysts which were first described byPhillipsen in 1956. Lesions are frequently multiple and a component of Nevoid Basal Cell CarcinomaSyndrome (NBCCS) (Gorlin Goltz syndrome/Bifid rib syndrome). We hereby report a case of multipleOKCs in a non – syndromic patient and highlight the general practitioner the importance of diagnosingthe disease and enforcing a strict long-term follow-up whenever such a case is identified.

Os keratocistos odontogênicos são cistos de desenvolvimento que foram descritos primeiramentepor Phillipsen, em 1956. As lesões são frequentemente múltiplas, sendo componentes da síndromedo carcinoma nevoide de células basais (síndrome de Gorlin, síndrome das costelas bífidas).Descreve-se um caso de keratocistos múltiplos em paciente não-sindrômico, enfatizando-se aimportância do clínico geral no diagnóstico da doença e reforçando a necessidade de umapreservação a longo prazo tão logo a doença seja diagnosticada.

The prevalence of oral mucosal lesions in patients visiting a dental school in Southern India.

The purpose of the present study was to evaluate the prevalence of oral mucosal lesions in Manipal, Karnataka State, India. A total of 1190 subjects who visited the department of oral medicine and radiology for diagnosis of various oral complaints over a period of 3 months were interviewed and clinically examined for oral mucosal lesions. The result showed the presence of one or more mucosal lesions in (41.2%) of the population. Fordyce's condition was observed most frequently (6.55%) followed by frictional keratosis (5.79%), fissured tongue (5.71%), leukoedema (3.78%), smoker's palate (2.77%), recurrent aphthae, oral submucous fibrosis (2.01%), oral malignancies (1.76%), leukoplakia (1.59%), median rhomboid glossitis (1.50%), candidiasis (1.3%), lichen planus (1.20%), varices (1.17%), traumatic ulcer and oral hairy leukoplakia (1.008%), denture stomatitis, geographic tongue, betel chewer's mucosa and irritational fibroma (0.84%), herpes labialis, angular cheilitis (0.58%), and mucocele (0.16%). Mucosal lesions like tobacco-related lesions (leukoplakia, smoker's palate, oral submucous fibrosis, and oral malignancies) were more prevalent among men than among women. Denture stomatitis, herpes labialis, and angular cheilitis occurred more frequently in the female population.

Central giant cell granuloma of the anterior maxilla.

Central giant cell granuloma (CGCG) formerly called giant cell reparative granuloma is a non-neoplastic proliferative lesion of unknown etiology. It occurs most commonly in the mandible. The case reported here resembled a wide variety of conditions that led to a misdiagnosis both on clinical and radiographic examination but was histopathologically diagnosed as CGCG. We describe a case of central giant cell granuloma arising from the anterior maxilla to highlight to the general dental practitioner the importance of histopathology in the diagnosis of this enigmatic lesion.

Down syndrome with transient myeloid leukemia and urological abnormality.

Among the various anomalies associated with Down syndrome, leukemia is quite common. The variant transient myeloid leukemia is seen almost exclusively in the Down syndrome patients. On the other hand, urological anomalies are infrequently found both in the Down syndrome and leukemia patients. We report a case who had the rare combination of a urological anomaly along with Down syndrome and transient myeloid leukemia.

Laparoscopy in diagnosis of prolonged neonatal jaundice.

When managing neonatal jaundice, there is no single test or imaging modality that can reliably define biliary atresia and neonatal hepatitis. Early diagnosis is an important step for surgical success in extra hepatic biliary atresia. In many situations, exploratory laparotomy and operative cholangiography may be needed to settle the definitive diagnosis, with the risk of having negative exploration in those high risk patients with medical etiology. The use of laparoscopy may help in avoiding unnecessary exploration for such group of patients and arriving at a definite diagnosis. Six patients with conjugated hyperbilirubinemia were evaluated with a diagnostic laparoscopy, laparoscopic cholangiography and liver biopsy. Three of the six patients were diagnosed to have neonatal hepatitis and so an unnecessary laparotomy was avoided in these cases.

Thoracoscopy in management of empyema thoracis in children.

The usual treatment for empyema in children varies from a simple thoracocentesis to thoracotomy and open decortication. We studied the role of thoracoscopy in the management of empyema thoracis in 10 immunocompetent children after failure of medical management. All children recovered well with an early removal of intercostal tube and reduced postoperative hospital stay and showed complete resolution of empyema on follow up. Thoracoscopy has come as a new ray of hope for the patients with empyema, with the advantages of complete evacuation, minimal pulmonary dysfunction, reduced pain and hospital stay.

Características clínico-epidemológicas do carcinoma epidermóide de cavidade oral no sexo feminino / Clinical and epidemiological characteristics of squamous cell carcinoma of oral cavity in women

OBJETIVO: Este trabalho tem como objetivo identificar as principais diferenças clínico-epidemiológicas do carcinoma epidermóide de cavidade oral no sexo feminino quando comparado ao sexo masculino. MÉTODOS: Foram estudados retrospectivamente os prontuários de 228 pacientes do sexo feminino portadoras de carcinoma epidermóide de cavidade oral, atendidos no Serviço de Cirurgia de Cabeça e Pescoço do Hospital Heliópolis, no período entre 1977 e 1996. As características clínico-epidemiológicas destas pacientes foram comparadas com aquelas de 849 pacientes do sexo masculino. RESULTADOS: A idade das pacientes das mulheres variou de 2 a 100 anos, com uma média de 60,7 anos. Nos homens encontramos idade entre 17 e 88 anos com uma média de 55,6 (p<0,00001). Quando o etilismo e o tabagismo estavam ausentes, a incidência do carcinoma epidermóide de boca ocorreu em uma faixa etária mais tardia. A presença do tabaco isoladamente näo afetou a distribuiçäo por faixa etária, enquanto que o álcool isoladamente ou em associaçäo com o tabaco leva a uma incidência mais precoce do câncer de boca. Os dois principais sítios de incidência säo a língua e o soalho. As mulheres têm uma menor incidência de lesöes de língua e soalho (43 por cento) quando comparado aos homens (61 por cento). Aproximadamente 50 por cento das mulheres eram estadio III e IV no momento do diagnóstico. Entre os homens este índice atinge 53 por cento. CONCLUSÖES: O carcinoma epidermóide de cavidade oral nas mulheres tem características clínico-epidemiológicas peculiares que justificam o desenvolvimento de protocolos de diagnóstico e tratamento específico para ele