Kidney-jing deficiency reduces the fertility of male mice and their male offspring / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the effects of kidney-jing deficiency on the fertility of male mice and their male offspring.</p><p><b>METHODS</b>Thirty 6-week-old Kunming male mice and 300 female ones were randomly allocated to a blank control group, a model group and a kidney-tonifying group. The model and the kidney-tonifying groups were stressed by fear plus excessive sex to establish a kidney-jing deficiency model, and meanwhile the latter were given concentrated solution of Kidney-tonifying Recipe intragastrically at the dose of 0.16 ml/10 g. The control and the model groups were treated with physiological saline at the same dose for 21 days. Then all the male mice were mated with the healthy estrous females for 5 days. The sperm density and motility of each group of the male mice were examined, and their fertility was assessed by comparison of the pregnancy rate and the number of baby mice at each birth among their female mates. And the sperm density and motility of the male offspring were detected at 6 weeks.</p><p><b>RESULTS</b>The average number of baby mice at each birth in the model group was (7.00 +/- 1.73), significantly smaller than those in the control (9.43 +/- 1.27) and the kidney-tonifying group (8.80 +/- 1.10) (P < 0.05). The sperm density and motility of the model mice were (9.70 +/- 1.15) x 10(6) / ml and (66.72 +/- 10. 12) %, lower than those of the control ([14.08 +/- 1.15 x 10(6)/ ml and [81.75 +/- 3.56] %), and the kidney-tonifying group ([12.20 +/- 1.55] x 10(6)/ ml and [78.55 +/- 4.38] %) (P < 0.05). There was no significant difference between the latter two groups (P > 0.05). The sperm density and motility of the offspring of the model mice were (10.10 +/- 1.79) x 10(6)/ ml and (71.86 +/- 7.48) %, lower than those of the control ([15.30 +/- 1.83] x 10(6)/ ml and [79.86 +/- 5.68] %), and the kidney-tonifying group ([14.20 +/- 2.21] x 10(6)/ ml and [81.92 +/- 2.51] %) (P < 0.05), with no significant difference between the latter two groups (P > 0.05).</p><p><b>CONCLUSION</b>Fear plus excessive sex could reduce the fertility of male mice and even that of their male offspring. And kidney-tonifying therapy could counteract this effect.</p>

Clinical observation on anovulatory infertility of blood and essence asthenia type treated by Lin'erlai Prescription / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the clinical effect of Lin'erlai Prescription (LEL) in treating anovulatory infertility of blood and essence asthenia syndrome type (BEAS).</p><p><b>METHODS</b>Sixty patients with anovulatory infertility in Western medical term, and classified as BEAS in Chinese medical term were equally assigned to two groups, they were treated with LEL (the treated group) and clomid (the control group) respectively. The ovulation rate and pregnant rate were observed.</p><p><b>RESULTS</b>The total effective rate in the treated group was 96.7%, which was significantly higher than that in the control group (53.3% , P < 0.05). The abortion rate was 10.0% in the treated group, significantly lower than that in the control group (54.6% , P<0.05); the TCM symptom score decreased significantly in the treated group after treatment (P < 0.01), but unchanged in the control group.</p><p><b>CONCLUSION</b>LEL has good effect in treating anovulatory infertility of BEAS type.</p>

Damage to and protection of sperm DNA / 中华男科学杂志

The damage to sperm DNA is one of the most important causes of male infertility. Some sperm with damaged DNA may escape from the sperm surveillance mechanism and transmit the damage to the offspring. So research on the damage to sperm DNA has become one of the hot spots in reproductive medicine. The factors that would damage sperm DNA include oxidative stress, microelements, reproductive toxic substances, radioactive rays, and so on, while the body depends on the compressed sperm DNA and anti-oxidation system for the protection of the integrity of sperm DNA. Some drugs such as anti-oxidant, black tea extract, etc, may help to improve and rebuild these protective mechanisms.

Clincal observation of qingre yulin decoction in treatment of male infertility caused by accessory gland infection / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effect of Qingre Yulin Decoction (QYD) on male infertility caused by accessory gland infection (AGI) with randomized controlled trial (RCT).</p><p><b>METHODS</b>Sixty infertility outpatients were equally divided into two groups randomly, the QYD group treated with modified QYD and the control group with antibiotic plus vitamin E, both for 3 months with another 6 months' follow-up. Pregnant rates, routine test of sperm and expressed prostatic secretion (EPS) were determined.</p><p><b>RESULTS</b>The healed rate was 26.7% (8 cases), the markedly effective rate was 43.3% (13 cases), the effective rate was 16.7% (5 cases), and the total effective rate was 86.7% in the QYD group, while in the control group it was 6.7% (2), 30.0% (9), 40.0% (12) and 76.7% respectively, showing higher healed rate and total effective rate in the former than those in the latter. Sperm quality of infertility patients with AGI decreased obviously, manifesting short ened average liquefaction time, reduced concentration, survival rate and vitality of sperm. These abnormal changes were improved after treatment in both groups, and the efficacy was better in the QYD group than that in the control group.</p><p><b>CONCLUSION</b>Infertility patients with AGI were manifested as oligospermatism and asthenospermia, which may not be the definite outcome of AGI. QYD is able to improve sperm quality, especially sperm vitality in infertility patients with AGI and therefore increase pregnant rate of their wives.</p>

Effects of experimental varicocele on the apoptosis of spermatogenic cells in rats / 中华男科学杂志

<p><b>OBJECTIVE</b>To explore the effects of experimental varicocele on the apoptosis of spermatogenic cells in rats.</p><p><b>METHODS</b>Thirty-two Sprague-Dawley(SD) male rats were randomly divided into four groups: pseudo-operation group, 45 d experimental group, 60 d experimental group and 90 d experimental group. Experimental varicocele was created by partial ligation of left renal vein. The apoptosis number of spermatogenic cells was measured by flow cytometry.</p><p><b>RESULTS</b>No marked apoptosis peak was observed in the control group, but it was observed in all the other groups, and the height of the peak increased with the duration of experiment.</p><p><b>CONCLUSIONS</b>Varicocele can induce apoptosis of large numbers of spermatogenic cells, and the decrease of spermatogenic cells. This may be the mechanism of varicocele causing male sterility.</p>