RESUMO
<p><b>OBJECTIVE</b>To confirm the role of Tiam1 (T lymphoma invasion and metastasis 1) gene in the proliferation and metastasis of colorectal cancer.</p><p><b>METHODS</b>Proliferative and metastatic abilities of Tiam1 transfectant were investigated by subcutaneous injection of cells and surgical orthotopic transplantation (SOI) in mice.</p><p><b>RESULTS</b>The expression of Tiam1 led to a pronounced increase in HT29/Tiam1 cell growth starting from day 7, up to 2.5 fold increase of tumor volume at day 20 post injection. Tumors in the HT29/Tiam1 group receiving surgical orthotopic implantation were significantly heavier than those in HT29/mock group (t = -14.916, P < 0.01). In vivo metastasis assay by SOI showed that in HT29/Tiam1 group, 7/7 of mice developed peritoneal metastases and 4/7 had hepatic lesions. In addition, one of the seven HT29/Tiam1 group mice had tumors in lung, spleen and lymph nodes. In the HT29/mock group, only 2/7 of animals had peritoneal metastases and none produced detectable tumor in the liver.</p><p><b>CONCLUSIONS</b>Tiam1 gene plays an important role in the proliferation, invasion and metastasis of colorectal cancer. It may serve as a useful clinical marker for tumor progression and metastasis of colorectal cancer.</p>
Assuntos
Animais , Humanos , Camundongos , Biomarcadores Tumorais , Proliferação de Células , Neoplasias Colorretais , Patologia , Fatores de Troca do Nucleotídeo Guanina , Genética , Metabolismo , Fisiologia , Células HT29 , Neoplasias Hepáticas , Neoplasias Pulmonares , Metástase Linfática , Camundongos Nus , Invasividade Neoplásica , Transplante de Neoplasias , Neoplasias Peritoneais , Plasmídeos , Proteína 1 Indutora de Invasão e Metástase de Linfoma de Células T , Transfecção , Carga TumoralRESUMO
<p><b>OBJECTIVE</b>To investigate whether the Tiam1 gene expression enhances the invasive and metastatic capabilities of colorectal carcinoma cells.</p><p><b>METHODS</b>Endogenous expression of Tiam1 in five colorectal carcinoma cell lines was investigated by RT-PCR. Tiam1/C1199HA cDNA was transfected into HT29, a colorectal carcinoma cell line without endogenous Tiam1 expression. RNA and protein expression of Tiam1 gene in the transfectants were detected by RT-PCR, immunohistochemistry and Western blot respectively. The biological behaviors of the transfectants were investigated by MTT and in-vitro invasion assays.</p><p><b>RESULTS</b>Tiam1 gene was highly expressed in LoVo and SW620 cells. Low level expression was seen in HCT116 and SW480 and no expression was found in HT29. Transfection of Tiam1 significantly increased the proliferation of HT29 cells along with markedly enhanced in-vitro invasion and metastasis.</p><p><b>CONCLUSIONS</b>Tiam1 gene plays an important role in the invasion and metastasis of colorectal carcinoma. It may be a useful marker for metastasis of colorectal carcinoma.</p>
Assuntos
Humanos , Biomarcadores Tumorais , Linhagem Celular Tumoral , Proliferação de Células , Neoplasias Colorretais , Genética , Metabolismo , Patologia , Regulação Neoplásica da Expressão Gênica , Fatores de Troca do Nucleotídeo Guanina , Genética , Metabolismo , Fisiologia , Células HT29 , Invasividade Neoplásica , Metástase Neoplásica , Proteína 1 Indutora de Invasão e Metástase de Linfoma de Células T , TransfecçãoRESUMO
Objective To investigate the clinical characteristics of colorectal cancer and its trend in Guangdong province.Methods The data from 6501 patients with pathologically confirmed colorectal cancer of 5 hospitals from 1990 to 2004 was analyzed.Results The mean age of 6501 patients with colo- rectal cancer was 59 years old,with the predominant age ranged from 61 to 70 years old.The proportion of young patients decreased from 7.1% to 3.5%.and the ratio between male and female was 1.47:1, which was gradually increased with increasing of age.The prevelence reached a high peak(60 years old) and dropped after that as a single peak curve.The ratio between rectal cancer and colon cancer was 1.1: 1,and the descending colon cancer accounted for 27.6%.With ageing,the rectal cancers decreased while the right hemi colon cancer increased gradually.Tubular adenocarinoma was predominant with his- tological typing,which accounted for 76.0%.The progressing stage(Dukes B,C,and D)was found in 93.9% of all cases.Conclusions The onset age of patients with colorectal cancer become close to west- ern country.The proportion of young patients are decreasing.The ratio between male and female dis- plays a single peak curve from rising to dropping.A tendency of decreasing percentage in rectal cancer and the gradual increasing in right hemi colon cancer emphasize the importance of colonoscopy in colorec- tal caner screening.