Allergy and uterine leiomyomas: cooperative interaction with ACP[1] genetic polymorphism

The possible association between allergy and neoplastic disorders has been the subject of many investigations but no general relationship has been determined. Little attention, however, has been paid to the possible role of allergy in the clinical manifestations of these diseases. In this study, the role of allergy in the susceptibility to uterine leiomyomas and in their growth was investigated. Interaction with ACP[1], a genetic polymorphism associated with the growth of leiomyomas, has been also considered. Two hundred and three White woman from the population of Rome hospitalized for symptomatic leiomyomas requiring surgical intervention have been studied. One hundred thirty eight healthy women have been considered as controls. Allergy has been evaluated by prick test. T-test for equality of means, analysis of variance and linear correlation analysis has been performed. The level of statistical significance was set at 0.05. The frequency of allergic manifestations in women with leiomyomas does not differ from healthy women. The dimension of leiomyomas is lower in allergic than in non allergic women [p=0.004]. The ACP[1] *B/*B genotype and allergy cooperate in lowering the dimension of leiomyomas; the proportion of woman with small leiomyomas [<10 percentile] is much higher in allergic women carrying the *B/*B genotype as compared to other women [p<0.001]. About 8% of variance of leiomyomas dimension is attributable to the joint effect of ACP[1] and allergy. Allergic women with high concentration of ACP[1] f isoform [*B/*B genotype] are protected from excessive leyomioma growth. If confirmed in other clinical settings, our observation may have practical importance in identifying women at risk of more severe clinical manifestations

study of adenylate kinase locus 1 [Ak[1]] genetic polymorphism in diabetic pregnancy

Previous studies suggest that adenylate kinase locus 1 [Ak[1]] has an important role in the control of blood glucose level and in the glycation of structural and functional proteins in type 2 diabetes and in the balanced development of fetoplacental unit in healthy puerperae [HP]. In this study, an attempt was made to investigate the relationship of Ak[1] with maternal and neonatal parameters in puerperae with gestational diabetes [GDP] and with preexisting type 1 diabetes [T1DP]. This study was carried on 402 HP, 347 consecutive healthy newborns, 102 GDP and 111 T1DP with their newborn infants. Ak[1] phenotype was determined by starch gel electrophoresis. Chi-square test of independence was carried out by SPSS program. The analysis of three way contingency table was carried out by a loglinear model. Significant level was 0.05. In T1DP, the frequency of Ak[1]*2 allele was higher than in GDP and in HP. Serum glucose level was higher in T1DP than in GDP with higher values in carriers of Ak[1]*2 allele. Neonatal hypoglycemia was more frequent in T1DP than in GDP with a positive association with Ak[1]*2 allele. The correlation between birth weight [BW] and placental weight [PW] was lower in infants from T1DP than HP. In healthy puerperae the correlation is higher in Ak[1] 2-1 than in Ak[1]1 phenotype while in diabetic puerperae the pattern is reversed with lower values in Ak[1]2-1 than in Ak[1]1 phenotype. The lowest value of correlation is observed in infants from T1D mothers carrying the Ak[1]*2 allele. The data confirmed the involvement of Ak[1] in glucose metabolism and showed a disturbance of the balance between placental and fetal growth which was more marked in T1DP

p53 Codon 72 Genetic Polymorphism in Asthmatic Children: Evidence of Interaction With Acid Phosphatase Locus 1

Several lines of evidence are implicating an increased persistence of apoptotic cells in patients with asthma. This is largely due to a combination of inhibition, or defects in the apoptotic process and/or impaired apoptotic cell removal mechanisms. Among apoptosis-inducing genes, an important role is played by p53. In the present study, we have investigated the possible relationship between p53 codon 72 polymorphism and asthma and the interaction with ACP1, a genetic polymorphism involved in the susceptibility to allergic asthma. We studied 125 asthmatic children and 123 healthy subjects from the Caucasian population of Central Italy. p53 codon 72 and ACP1 polymorphisms were evaluated using a restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) method. There is a statistically significant association between p53 codon 72 polymorphism and allergic asthma: Arg/Arg genotype is more represented in asthmatic patients than in controls (P=0.018). This association, however, is present in subjects with low ACP1 activity A/A and A/B only (P=0.023). The proportion of children with A/A and A/B genotype carrying Arg/Arg genotype is significantly high in asthmatic children than in controls (OR=1.941, 95% C.I. 1.042-3.628). Our finding could have important clinical implications since the subjects with A/A and A/B genotypes of ACP1 carrying Arg/Arg genotype are more susceptible to allergic asthma than Pro/Pro genotype.