RESUMO
A liquid chromatographic procedure was developed for the determination of a new anticancer agent Bicalutamide in plasma using fluorescence detection by using beta-Cyclodextrin as an enhancer. Chromatography was performed using a reversed phase trimethylsilyl bonded silica column with a mobile phase of water: acetonitrile [30:70] at a flow rate of 1 ml/min. Detection of the eluted peaks was observed using excitation and emission wavelengths of 272 and 328 nm, respectively. Chromatographic run lime did not exceed 10 minutes with no interference of endogenous material. The calibration curve was linear over the concentration range of 0.1 to 0.35 ug/ml and inter- and intra-assay imprecision was less than 10%. The lower limit of quantitation was assessed at 3.96X10 [-5] microg/ml, 1.01X10 [-4] microg/ml and lower limit of detection was 1.3X10[-5] microg/ml, 3.34X10 [-5] microg/ml in formulation and biological fluids respectively