RESUMO
Increasing evidences link free radicals and oxidative stress to the pathogenesis of diabetes and related complications. The aim of this study was to investigate the effects of exogenous coenzyme Q10 supplementation on streptozotocin induced experimental diabetes in rats. Experimental diabetes was induced by single subcutaneous injection of streptozotocin ( 50 mg/kg/ body weight) . Forty male albino rats were used in this study, they were divided into 4 groups: control group, coenzyme Q10 treated group, diabetic group and coenzyme Q10 treated diabetic group. At the end of the experiment (8 weeks) urine samples were collected, rats were sacrificed and blood samples were collected for biochemical analysis. Significant high levels of blood sugar, glycated Hb, total cholesterol, triglyceride, LDL cholesterol, advanced oxidative protein products , 8-hydroxy-2-deoxyguanosine and percent of DNA damage as well as significant low levels of nitric oxide and HDL cholesterol were observed in diabetic group. Significant low levels of blood sugar, glycated Hb, cholesterol, triglyceride, LDL cholesterol, advanced oxidative protein products, 8-hydroxy-2-deoxyguanosine and percent of DNA damage as well as significant high levels of nitric oxide and HDL cholesterol were observed in coenzyme Q10 treated diabetic group. The data confirmed the property of coenzyme Q10 as an antioxidant that ameliorates oxidative stress and that it may be used as an additional therapeutic agent for prophylaxis and slowing down the progression diabetic complications.
RESUMO
The present study was conducted to evaluate the antiatherogenic effect of almond oil in diabetic rats. Forty five male white albino rats were divided into 3 groups: Control group, diabetic group and diabetic almond oil treated group. Experimental diabetes was induced by single subcutaneous injection of 50mg/kg body weight streptozotocin. After two months blood samples were collected, for assessment of triglycerides, total, HDL, and LDL cholesterol, insulin, intercellular adhesion molecule-1 (ICAM-1), nitrite and nitrate (NOx), hydrogen peroxide (H2O2), glutathione peroxidase activity (GPX) and DNA damage. Results showed that mean levels of cholesterol, triglyceride, LDL-cholesterol were significantly low and HDL-cholesterol was significantly high in diabetic group received almond oil compared to diabetic group. Mean concentrations of insulin, NOx , GPX activity were significantly high, and mean levels of H2O2 , ICAM-1, percent of DNA damage were significantly low in diabetic group received almond oil compared to diabetic group . The data confirmed property of almond oil as an antioxidant that ameliorates oxidative stress and revealed that it is efficiently improves endothelial function and protects against the development of atherosclerosis in STZ induced diabetic rats.