Tumor carcinoide na vesícula biliar em cão / Carcinoid gallbladder tumor in a dog

Carcinoid is a neoplasia that arises from dispersed cells of the neuroendocrine system. This tumor is uncommon in animals, and its occurrence in the gallbladder is rare. A male Basset Hound dog's corpse was taken to the Univerdade Federal de Minas Gerais to be analyzed by the Veterinary Pathology sector, without a description of its previous history. Necropsy revealed the presence of pale oral, ocular and penile mucous membranes. The gallbladder had a thickened wall and a dilated lumen, which was filled with dark and lumpy bile. Its mucosa had a whitish-red nodule, with solid and friable areas. Microscopically, there was a focal neoplastic proliferation, which wasn't encapsulated and had imprecise limits, which cells were distributed in a solid pattern and separated by a delicate fibrovascular stroma. The neoplastic cells presented oval or round shaped nucleus, which had a chromatin predominantly loose, and one or two nucleoli. Their cytoplasm was moderately abundant, and in most of the cells it was eosinophilic, granular, and had well-defined limits. Using the Grimelius coloration, neoplastic cells' cytoplasmic granules stained brownish or black, confirming the neuroendocrine origin of the neoplasia. Based on the macroscopic and microscopic findings, the diagnosis of a gallbladder carcinoid was established.(AU)

Modelo de indução de osteoporose em coelhas - avaliação radiográfica / Rabbit osteoporosis induction model - radiographic evaluation

In order to establish a bone loss induction model in rabbits to study osteoporosis, 18 rabbits underwent ovariectomy and received methylprednisolone 1mg/kg intramuscularly on alternate days for two weeks. Immediately before ovariectomy up to 42 days after methylprednisolone administration, radiographs of the right olecranon were taken in the mediolateral position to evaluate the radiographic optical density. Before the induction of osteoporosis, rabbits presented mean values of radiographic density of 2.305mm Al and at 42 days of 1.575mm Al. The values obtained were submitted to ANOVA for repeated measures that revealed a significant drop (P< 0.001) in the density over time, proving that the induction was able to trigger bone loss of these animals. With this, it can be affirmed that the adopted protocol was enough to provoke a significant bone loss, characterizing a valid model for the study of new treatments for osteoporosis.(AU)

Humane endpoint in mice by Brazilian researchers in the vaccine sector / Ponto final humanitário em camundongos aplicado por pesquisadores brasileiros da área de vacinas

The application of a humane endpoint (HE) for mice in vaccine trials and further challenging tasks with lethal samples is necessary to reduce or prevent pain and suffering in these animals, and is a refinement of the 3R policy enforced for animal testing in both national and international scenarios. In order to investigate the application of HE in Brazil, researchers from the vaccine sector have answered a questionnaire about their research profile, their usage of HE and their knowledge of its defining criteria, monitoring of animals, staff training, and euthanasia methods employed. The main results revealed that researchers failed to recognise the very concept of HE as well as when to apply it. In addition, the Institutional Animal Care and Use Committees (IACUCs) failed upon giving their approval to the trials. HE is an important refinament of animal testing policy, and these results highlight the need for a clear pre-established definition of when and how it should be implemented in order to ensure more effective application. Furthermore, it is important to clarify the ethics involved and the commitment of both the research teams and IACUCs to animal welfare.(AU)

A aplicação de um ponto final humanitário (PFH) em experimentos com vacinação e posterior desafio com amostras letais em camundongos é necessária para diminuir ou impedir a dor e o sofrimento desses animais, constituindo refinamento de acordo com a política dos 3Rs, vigente em âmbito nacional e internacional, no cenário da experimentação animal. A fim de investigar a aplicação de PFH no Brasil, foi enviado um questionário para pesquisadores da área de vacinas, com perguntas sobre perfil do pesquisador, aplicação de PFH e critérios para sua definição, monitoramento dos animais, treinamento da equipe e métodos de eutanásia empregados. Os principais resultados revelaram que há falhas tanto em reconhecer o conceito quanto em aplicar o PFH, por parte dos pesquisadores, e que as CEUAs também falharam ao aprovar tais experimentos. Chama-se atenção para o fato de que o PFH é um importante refinamento e para a necessidade de definição prévia de PFH e sua aplicação, assim como para a importância do esclarecimento da questão ética envolvida e do necessário compromisso das equipes de pesquisa e das CEUAs com o bem-estar animal.(AU)

Action of phosphorylated mannanoligosaccharides on immune and hematological responses and fecal consistency of dogs experimentally infected with enteropathogenic Escherichia coli strains

The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of blood cells in the hemogram and IgA and IgG production. MOS was effective in recovering of EPEC-infected dogs since prebiotic-treated animals recovered more rapidly from infection than untreated ones (p < 0.05). This is an important finding since diarrhea causes intense dehydration and nutrient loss. The use of prebiotics for humans and other animals with diarrhea can be an alternative for the treatment and prophylaxis of EPEC infections.

Molecular detection of anaplasma platys in a naturally-infected cat in Brazil

Following the accidental finding of inclusion bodies similar to Anaplasma platys in a stained blood smear from a cat, DNA analysis of the 16S rRNA gene was performed and 100 percent identity was found with different strains of A. platys. These data confirm that cats are susceptible to parasitism by A. platys.

Parâmetros genéticos para características de carcaça avaliadas por ultrassonografia em bovinos da raça Guzerá / Genetic parameters for body weight and real-time ultra sound carcass traits of Guzera cattle

A partir das observações de 1.325 animais (90,4 por cento de machos e 9,6 por cento de fêmeas) e do pedigree de 6.642 animais da raça Guzerá foram estimados os parâmetros genéticos para o peso corporal e as características área de olho de lombo e espessura de gordura na costela e na garupa, avaliadas por meio da técnica de ultrassonografia. Os componentes de (co)variância foram estimados pelo método da máxima verossimilhança restrita, utilizando-se o aplicativo MTDFREML. Foram utilizados, para as estimativas de repetibilidade e herdabilidade, modelos unicaracterística e, para as correlações genéticas e fenotípicas entre as características, modelos bicaracterísticas. As estimativas de repetibilidade (erros-padrão) foram 0,44(0,10) para peso corporal, 0,39(0,10) para área de olho de lombo, 0,75(0,06) para espessura de gordura na costela e 0,49(0,08) para espessura de gordura na garupa. As estimativas de herdabilidade, respectivamente a partir de modelos uni e bicaracterísticas, foram 0,42(0,11) e 0,41(0,11) para peso corporal, 0,35(0,09) e 0,34(0,09) para área de olho de lombo, 0,20(0,08) e 0,32(0,02) para espessura de gordura na garupa e 0,05(0,06) e 0,10(0,08) para espessura de gordura na costela. As estimativas de correlações genéticas foram 0,79(0,09) entre o peso corporal e a área de olho de lombo; 0,20(0,08) entre o peso corporal e a espessura de gordura na garupa; 0,05(0,06) entre a área de olho de lombo e a espessura de gordura na costela; 0,02(0,27) entre a área de olho de lombo e a espessura de gordura na garupa; e 0,64(0,22) entre as duas medidas de espessura de gordura. Os resultados indicam que é uma mensuração suficiente para a adequada avaliação das características área de olho de lombo e espessura de gordura na carcaça e que a seleção direta para essas características pode resultar em carcaças mais musculosas e de melhor acabamento. Indica, ainda, ausência de antagonismo genético entre a seleção para peso corporal e características ...

Genetic parameters were estimated for body weight and real-time ultra-sound loin-eye area, rump fat thickness, and back fat thickness using data from 1,325 yearling Guzera cattle (90.4 percent bulls and 9.6 percent heifers) and pedigree structure with 6,642 animals. Variance and covariance components were estimated using REML methodology and MTDFREML software. Single trait animal models were used to estimate repeatability and heritability for the four traits. Multiple traits animal models were used to estimate genetic correlations among the traits. Repeatability estimates (standard errors) were 0.44(0.10) for body weight, 0.39(0.10) for loin-eye area, 0.75(0.06) for rump fat thickness, and 0.49(0.08) for back fat thickness. Heritability estimates were 0.42(0.11) and 0.41(0.11) for body weight, 0.35(0.09) and 0.34(0.09) for loin-eye area, 0.20(0.08) and 0.32(0.08) for back fat thickness, and 0.05(0.06) and 0.10(0.08) for rump fat thickness, respectively from single and multiple traits models. Genetic correlation estimates were 0.79(0.09) between body weight and loin-eye area, 0.20(0.08) between body weight and back fat thickness, 0.05(0.06) between loin-eye area and rump fat thickness, 0.02(0.27) between loin-eye area and back fat thickness and 0.64(0.22) between the two measurements of fat thickness. In order to evaluate carcass traits, results suggested that repeated real-time ultra-sound measurements are not needed and that direct selection for these traits might be effective. In addition, there is no genetic antagonism between selection for body weight and carcass traits.

Normal microbiota of the perialveolar region of incisors of rats / Microbiota normal da região perialveolar de incisivos em ratos

Identificou-se a microbiota normal da região peri-alveolar de incisivos em 72 ratos Wistar, com 70-90 dias de idade e 280-330g de peso. As bactérias foram coletadas com suabes embebidos em solução salina. Do material depositado em tubo contendo 460µl de Brain Heart Infusion e diluído em 1:10, retirou-se 1µl para semeadura em placas de Petri. O crescimento médio foi 1,4x10(6) ± 2,6x10(5)UFC/ml. Segundo a ordem das freqüências, as bactérias encontradas foram: Bacillus sp., Staphylococcus aureus, Streptococcus viridans, Corynebacterium sp., Staphylococcus coagulase negativa, Enterococcus sp., Staphylococcus saprophyticcus, Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae e Serratia liquefaciens. De todas as bactérias isoladas, as Gram-positivas e as Gram-negativas compreenderam 91,2 por cento e 8,8 por cento, respectivamente. Dentre as Gram-positivas, a mais freqüente foi Bacillus sp.(31,2 por cento) e a menos, Staphylococcus saprophyticcus (3,0 por cento). Quanto às bactérias Gram-negativas, a mais encontrada foi Escherichia coli (50,1 por cento) e a menos, Serratia liquefaciens (6,2 por cento)

Freqüencia de anticorpos anti-Anaplasma marginale em rebanhos leiteiros da Bahia / Frequency of antibodies against Anaplasma marginale in dairy herds of Bahia State, Brazil

Determinou-se a freqüência de bovinos soro-reagentes para Anaplasma marginale em rebanhos leiteiros das microrregiöes de Jequié, Itabuna e Vitória da Conquista, Estado da Bahia, pelas técnicas de imunoadsorçäo enzimática (ELISA), imunofluorescência indireta (IFI) e teste de conglutinaçäo rápida (TCR). Das 324 amostras de soro bovino analisadas, 96,9 por cento, 97,2 por cento e 91,0 por cento foram positivas para anticorpos contra A. marginale, respectivamente pelo ELISA, IFI e TCR. Todas as regiöes caracterizaram-se por estabilidade enzoótica para a anaplasmose. O desempenho dos três testes sorológicos foi bastante similar na detecçäo de anticorpos contra A. marginale