RESUMO
Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells [ESCs] proliferation and their expression of adiponectin receptors
Methods: In this experimental study, endometrial biopsies [n=7] were taken. ESCs isolation was done by enzymatic digestion and cell filtrations. ESCs of each biopsy were divided into four groups: 0 [control], 10, 100, and 200 ng/ml adiponectin concentrations in three different times [24, 48, or 72 h]. The effect of adiponectin on ESC viability and expression of mRNA Adipo receptorl [Rl] and Adipo receptor2 [R2] was determined by Trypan blue staining and semi-quantitative RT-PCR, respectively. Data were analyzed by one-way ANOVA and unpaired student's t-test, and P<0.05 was considered statistically significant
Results: Adiponectin inhibited human endometriotic stromal cell proliferation in time- and dose-dependent manners significantly [P=0.001]. Expression of AdipoRl and AdipoR2 gene receptors was increased in human ESCs significantly [P<0.05]. Adiponectin can suppress endometriosis by inhibiting ESC proliferation and increased AdipoRl and AdipoR2 expression
RESUMO
Background: Toxic effects of anti-cancer and other drugs on the normal tissues could be reduced by the herbal plants and their fractions. This study investigated the protective effect of thymoquinone [TQ] as a fraction of Nigella sativa on methotrexate [MTX]- induced germ cell apoptosis in male mice
Materials and Methods: In this experimental study, thirty male Balb/c mice were divided randomly into 5 groups [n=6]. A single dose of MTX [20 mg/kg] and different concentrations of TQ were administrated for 4 consecutive days. Terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL] assay was performed on paraffin embedded tissue sections to analysis the occurrence of apoptosis in the testis. Reverse transcription polymerase chain reaction [RT-PCR] of apoptosis-related genes was performed with RNA extracted from testes of the mice. Statistical analysis was done using one-way ANOVA
Results: In the MTX group, there was a significant increase in morphologic sign of germ cell degeneration of tubules [48 +/- 0.6%], apoptotic index [AI; 2.3 +/- 0.6%], as well as mRNA expression of p53 [P=0.008], caspase 8 [P=0.002], caspase 3 [P=0.005], caspase 9 [P=0.000], bax [P=0.004] and the ratio of bax/bcl-2 [P=0.000], whereas there was an decrease in the expression of bcl-2 [P=0.003], as compared to control group. In MTX+TQ groups, the data showed that different concentrations of TQ could improve the harmful effects caused by the MTX. The best protective effects were achieved in MTX+TQ [10 mg/kg]
Conclusion: TQ protects testicular germ cell against MTX-induced apoptosis by affecting related genes regulation