Influence of Smokeless Tobacco on Periodontal Health Status in Local Population of North India: A Cross-Sectional Study

Introduction: In addition to smoking, smokeless tobacco hasalso evidently shown its effect on various oral tissues.Smokeless tobacco products have been in existence forthousands of years among populations in South America andSoutheast Asia in varied forms.Methodology: This cross-sectional study investigated theeffect of commonly used smokeless tobacco on periodontalhealth in local population of nearby villages of Air Force StationBakshi Ka Talab, U.P., India, for which 2045 individuals[males=1455 (71%), females=590 (28.9%)] were evaluated.Results: Maximum incidence of smokeless tobaccoconsumption was observed in the age group 21–30 years(39.4%), whereas minimum consumption was observed in theage group 10–20 years (7.5%). The peak smokeless tobaccoconsumption was observed between the ages 21 and 40 years.Recording of oral hygiene measures included in this studyshowed majority of subjects (n=1961) used tooth brushing asthe oral hygiene measure, while some used datoon (n=30;1.5%) for cleaning their teeth and others used their finger(n=54; 2.6%) with or without tooth powder.Conclusion: This study conclude that, PPD were significantlyhigher statistically amongst longer duration (>5 years)smokeless tobacco users as compared to non-tobacco users.

Comparing Refractive Error with Axial Length in the Subjects Having Refractive Error

Introduction: Refractive error has now become the secondmost common cause of blindness across the world. Thereis necessity to explore the various factors having impact onrefractive errors as they will certainly assist in the variousmodes of treatment aspect of the refractive errors. This studywas carried out with the aim of comparing refractive errorwith axial length in the subjects with refractive error.Material and methods: In this study, total of 500 adult caseswere recruited. So total of 1000 eyes were examined upon.This study was carried out, over a total period of 2 years.General examination was carried out in each and every case.Afterwards, local examination was done. Then refractive errorand axial length was measured. The data obtained from thisstudy was analysed statistically.Results: The results of our present study have showed thatthe spherical refractive error has statistically highly significantnegative correlation with axial length, in right eye (r=-0.836,p<0.01) and in left eye (r=-0.859, p<0.01).Conclusion: The observation made from this study says thaton progressing from myopic to hyperopic refractive error, theaxial length of the eye is found to decrease. In the refractiveerror of shortsightedness or myopia, the final image is formedahead of retina

Detection of multi-drug resistance & characterization of mutations in Mycobacterium tuberculosis isolates from North- Eastern States of India using GenoType MTBDRplus assay.

Background & objectives: information on drug resistance tuberculosis is sparse from North-East (N-E) States of Iindia. We undertook this study to detect multi-drug resistant tuberculosis (MDR-TB) among MDR-TB suspects, and common mutations among MDR-TB cases using GenoType MTBDRplus. Methods: All MDR suspect patients deposited sputum samples to peripheral designated microscopy centres (DMC) in North-East States. The district TB officers (DTOs) facilitated the transport of samples collected during January 2012 to August 2012 to our laboratory. The line probe assay to detect common mutations in the rpoB gene for rifampicin (RIiF) and katG and inhA genes for isoniazid (IiNH), respectively was performed on 339 samples or cultures. Results: A total of 553 sputum samples from MDR suspects were received of which, 181 (32.7%) isolates were found to be multi-drug resistant. Missing WT8 along with mutation in codon S531L was commonest pattern for rifampicin resistant isolates (65.1%) and missing WT along with mutations in codon S315T1 of katG gene was commonest pattern for isoniazid resistant isolates (86.2%). Average turn-around time for dispatch of LPA result to these sStates from cultures and samples was 23.4 and 5.2 days, respectively. Interpretations & conclusions: The MDR-TB among MDR-TB suspects in North-Eastern States of Iindia was found to be 32.7 per cent. The common mutations obtained for RIiF and IiNH in the region were mostly similar to those reported earlier.

Unusual topography of posterior antebrachial musculature in the first osseofibrous compartment of wrist: clinicomorphological appraisal / Topografía inusual de la musculatura antebraquial posterior en el primer compartimento osteofibroso de la muñeca: valoración clínico-morfológica

Anatomical variations of abductor pollicis longus and extensor pollicis brevis are important in clinical assessment of diseased and traumatized hand. The present case reports an unusual fusion of muscle bellies of abductor pollicis longus and extensor pollicis brevis with two separate tendons of insertion, the medial tendon inserted into the base of first metacarpal and the lateral tendon into the abductor pollicis brevis muscle. Knowledge of such anatomical variations is of utmost importance in the management of De Quervain's disease and reconstructive surgeries of hand.

Las variaciones anatómicas de los músculos abductor largo del pulgar y extensor corto del pulgar son importantes en la evaluación clínica de la mano enferma y traumatizada. El presente caso informa una inusual fusión de los vientres musculares de los Mm. abductor largo del pulgar y extensor corto del pulgar con dos tendones de inserción separados, el tendón medial se insertó en la base del primer metacarpiano y el tendón lateral en el músculo abductor corto del pulgar. El conocimiento de estas variaciones anatómicas es de importancia en el manejo de la enfermedad de De Quervain y cirugía reconstructiva de la mano.

Effect of maternal Tp53 gene G412C polymorphism on neural tube defects: A study from North India.

Context: Tumor protein 53 (tp53) is one of the candidate gene proposed for neural tube defects, which affects central nervous system during early embryonic development, on the basis of mouse models. Aims: The present study is an attempt to unfold the possible role of tp53 G412C polymorphism in the incidence of neural tube defect (NTDs) in humans. Settings and Design: Case-control study was carried out in government hospitals of Delhi, India. Materials and Methods: Subjects comprised of 100 mothers of NTD children and 100 matched control mothers. Information on some environmental exposures was collected along with blood samples. After DNA extraction, the genotyping of tp53 G412C polymorphism was carried out by PCR-RFLP method. Statistical Analysys: Fisher Exact or Chi square test, binary logistic model, and odds ratio (95% confidence interval) calculations were used to evaluate effect of risk factors on NTDs using SPSS v17.0. Results: The 'CC' genotype of tp53 G412C showed protective effect towards the development of anencephaly and/or encephalocele (OR: 0.44; 95% CI: 0.19-1.00); however, no significant difference among overall NTD cases and controls was observed (P>0.05). Further segregation of all subjects based on 2 different communities, Hindus and Muslims, the association of 'CC' genotype of the polymorphism with reduced NTD risk was observed among Hindu community (OR: 0.33; 95% CI: 0.13-0.79). Conclusion: The study highlights the selective advantage provided by maternal 'CC' genotype, thereby reducing risk of cephalic NTDs, probably due to the lower apoptotic activity of the protein, however, more specifically in the presence of community-specific microenvironment.

Fate of sputum samples transported in bottles containing Cetylpyridinium Chloride and Sodium Chloride: A National Reference Laboratory Study.

The transportation of sputum samples may sometimes take more than one week which results in an increased contamination rate and loss of positive cultures. The current study was planned to analyze the recovery rate of mycobacteria from transported samples with and without Cetylpyridinium chloride (CPC). Addition of CPC is useful for isolation of M. tuberculosis from sputum subjected to long-term storage.

Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India

The aim of the present study was to compare polymerase chain reaction (PCR)-based methods - spoligotyping and mycobacterial interspersed repetitive units (MIRU) typing - with the gold-standard IS6110 restriction fragment length polymorphism (RFLP) analysis in 101 isolates of Mycobacterium tuberculosis to determine the genetic diversity of M. tuberculosis clinical isolates from Delhi, North India. Spoligotyping resulted in 49 patterns (14 clusters); the largest cluster was composed of Spoligotype International Types (SITs)26 [Central-Asian (CAS)1-Delhi lineage], followed by SIT11 [East-African-Indian (EAI) 3-Indian lineage]. A large number of isolates (75 percent) belonged to genotypic lineages, such as CAS, EAI and Manu, with a high specificity for the Indian subcontinent, emphasising the complex diversity of the phylogenetically coherent M. tuberculosis in North India. MIRU typing, using 11 discriminatory loci, was able to distinguish between all but two strains based on individual patterns. IS6110-RFLP analysis (n = 80 strains) resulted in 67 unique isolates and four clusters containing 13 strains. MIRUs discriminated all 13 strains, whereas spoligotyping discriminated 11 strains. Our results validate the use of PCR-based molecular typing of M. tuberculosis using repetitive elements in Indian isolates and demonstrate the usefulness of MIRUs for discriminating low-IS6110-copy isolates, which accounted for more than one-fifth of the strains in the present study.

Unilateral quadripartite composition of pectoral major muscle: anatomical description and clinical implications / Composición unilateral cuadripartita del músculo pectoral mayor: descripción anatómica e implicancias clínicas

A unilateral anomalous disposition of the muscular slips of pectoralis major was observed in an adult male cadaver during routine gross anatomy teaching session. The pectoralis major muscle displayed unusual configuration and comprised of four parts delineated by three intramuscular clefts. Further, the sternocostal part was found to divide into two fasciculi. The origin and insertion of pectoralis major muscle was as usual and unremarkable. Interestingly, the innervation of the muscle was observed to bear an important variation. The clavicular and sternocostal slips of pectoralis major muscle received a direct branch from the lateral cord of brachial plexus in addition to its usual innervation from the lateral and medial pectoral nerves. The multiplicity of innervation of pectoralis major as encountered in the present study would presumably augment its suitability for its use in reconstructive procedures. An additional anomalous attachment of the pectoralis minor muscle was also observed emanating from the second rib, apart from the usual third, fourth and fifth ribs. These variants of the pectoral muscles may also have implications in biomechanical studies, as they may alter the kinematics.

Una disposición anómala unilateral de los vientres musculares del músculo pectoral mayor se observó en un cadáver adulto de sexo masculino durante una clase de rutina de anatomía. El músculo pectoral mayor mostró una configuración inusual y presentaba cuatro partes delimitadas por tres depresiones intramusculares. Además, la parte esternocostal se encontraba dividdida en dos fascículos. El origen y la inserción del músculo pectoral mayor era normal. Curiosamente, la inervación de los músculos, presentaba una importante variación anatómica. Los fascículos clavicular y esternocostal del músculo pectoral mayor recibieron una rama directa del fascículo lateral del plexo braquial, además de su inervación normal de los nervios pectoral lateral y medial. La múltiple inervación del músculo pectoral mayor como se encuentró en el presente estudio, probablemente, haga aumentar su idoneidad para el uso en procedimientos reconstructivos. También se observó un fascículo adjunto anómalo adicional del músculo pectoral menor, que se originaba de la segunda costilla, además de su origen en la tercera, cuarta y quinta costillas. Estas variaciones de los músculos pectorales también puede tener implicancias en estudios biomecánicos, ya que probablemente alteran la cinemática.

Unilateral double axillary and double brachial arteries: embryological basis and clinical implications / Arterias Axilar y Braquial Dobles Unilaterales. Bases Embriológicas e Implicaciones Clínicas

Este reporte describe la presencia de las arterias axilar y braquial duplicadas en el lado derecho de un cadáver adulto de sexo masculino. La arteria axilar derecha se bifurcó en arterias axilares I y II, localizadas posteriormente al músculo pectoral menor. Ambas arterias, con sus respectivas ramas atravesaron bajo el músculo redondo mayor y continuaron como arterias braquiales I y II, respectivamente. La arteria axilar I, que se continuó como arteria braquial I, tuvo un trayecto superficial y tortuoso, mientras que la arteria axilar II fue de poco calibre y de localización profunda. Las arterias toracoacromial, torácica lateral y subescapular se originaron de la arteria axilar I. La arteria torácica superior se originó de la arteria toracoacromial. Las arterias circunflejas humerales anterior y posterior se originaron desde la arteria axilar II. La arteria braquial profundase originó de la arteria braquial II, mientras que a nivel de la línea intercondílea, la arteria ulnar se originó de la arteria braquial I. En la fosa cubital, la arteria braquial I se dividió en arterias radial e interósea común, mientras que la arteria braquial II terminó anastomosándose con la arteria braquial I. Las arterias ulnar, radial e interóseas común continuaron como arterias independientes y tuvieron un trayecto y distribución normales en el antebrazo. La persistencia de arterias axilar y braquial dobles tiene una base embriológica, pudiendo ser de interés para la embriología experimental. El conocimiento de estas variaciones arteriales múltiples, como el presente caso, es de importancia fundamental durante la medición de flujo en los vasos sanguíneos a través de Doppler, para el diagnóstico clínico y manejo quirúrgico de enfermedades que afectan al miembro superior.

This report presents double axillary and double brachial arteries on the right side of an adult male cadaver. In this case, the right axillary artery bifurcated into axillary artery I and axillary artery II posterior to the pectoralis minor muscle. Both the axillary arteries with their branches traversed upto lower border of teres major muscle and continued further as seperate entities into the cubital fossa as brachial artery I and brachial artery II respectively. The axillary artery I which continued as brachial artery I was superficial and tortuous in its course, while the axillary artery II was slender and deeply situated. The thoraco-acromial, lateral thoracic and subscapular arteries took origin from axillary artery I. The superior thoracic artery was seen arising from the thoraco-acromial artery. The anterior and posterior circumflex humeral arteries arose from axillary artery II.The profunda brachii artery was given off by the brachial artery II, while at the level of intercondylar line, the ulnar artery was given off by brachial artery I. In the cubital fossa, the brachial artery divided into radial and common interosseous artery, while the brachial artery II ended by anastomosis with brachial artery I. The ulnar, radial and common interosseous arteries continued as separate entities and exhibited a normal course and distribution in the forearm.The hypothesis for the embryological basis of persistence of double axillary and double brachical arteries may be useful for experimental embryology. The knowledge of such multiple arterial variations as in the present case is of immense significance during Doppler scanning of blood vessels for clinical diagnosis and surgical management of diseases of superior extremity.

Effect of oil massage on growth and neurobehavior in very low birth weight preterm neonates.

OBJECTIVE: To study the effect of oil massage on growth and neurobehavior in preterm babies less than 1500 g. DESIGN: Randomized Controlled Trial. SETTING: Tertiary level neonatal unit of a teaching hospital. Subjects: Neonates with birth weight <1500 grams, gestation >37 weeks, receiving enteral feeds of at least 100 mL/kg/day and less than 10 days of age. OUTCOME VARIABLES: Primary-Weight gain 28 days after enrolment. Secondary-Neonatal neurobehavior, change in other anthropometry and serum triglycerides. METHODS: Eligible neonates were randomized to one of the three groups (a) massage with oil (b) massage without oil and (c) no massage. Weight, length, head circumference and triceps skin fold thickness were measured in the three groups at regular intervals. Serum triglyceride levels were measured at enrolment and at completion. Neurobehavior using Brazeltons Neonatal Behavior Assessment Scale (NBAS) was assessed at enrolment and after 10 days of intervention. RESULTS: Weight gain in the oil massage group (365.8 +/- 165.2g) was higher compared to the only massage group (290.0 +/- 150.2g) and no massage group (285.0 +/- 170.4g). This difference and the difference in other anthropometric parameters was not statistically significant. Serum triglycerides and neonatal neurobehavior were comparable in the three groups. CONCLUSION: Oil application may have a potential to improve weight gain among preterm very low birth weight neonates.