RESUMO
Using serial antigenic challenge as the method of selection and stimulation, continuous lines of cytotoxic T-lymphocytes (CTL) directed against TNBS-modified syngeneic spleen cells (TNP-self) have been generated. Spleen cells from C3H/HeJ (H-2k) mice were primed in vitro with autologous spleen cells modified with TNBS, and subsequently cloned by limiting dilution and in soft agar in the presence of IL2. These CTL clones grew continuously in medium supplemented with IL2 and in the presence of antigen. They are antigen specific and H-2 restricted in their target cell recognition. They all express Thyl and Lyt2 surface markers. None of the clones exhibit natural killer (NK) cell activity. All CTL clones tested so far are restricted in their target cell recognition to H-2Kk-TNP and none were found to be restricted to H-2Dk-TNP. These findings demonstrate at the clonal level the H-2K/D restriction of TNP-self specific CTL. These clones provide tools that may facilitate an understanding of the development and regulation of antigen specific CTL. They may also serve as models useful towards an understanding of the mechanism of lysis by CTL.
Assuntos
Animais , Células Clonais/imunologia , Epitopos , Antígenos H-2/imunologia , Camundongos , Camundongos Endogâmicos C3H , Nitrobenzenos/imunologia , Linfócitos T Citotóxicos/imunologia , Trinitrobenzenos/imunologiaRESUMO
Peritoneal encapsulation is a rare congenital anomaly. Its occurrence in a female and association with intestinal obstruction are even rarer features which were present in the reported case.
Assuntos
Feminino , Humanos , Íleo/anormalidades , Obstrução Intestinal/etiologia , Intestino Delgado/anormalidades , Pessoa de Meia-Idade , Peritônio/anormalidadesRESUMO
A rare case of linear scleroderma with plaques of morphea in hemicorporeal distribution and subclinical systemic involvement is presented. Its possibility of progression into systemic sclerosis, which has not been reported earlier, is discussed.