RESUMO
Aims: Hepatocyte transplantation is an alternative to liver transplantation for acute liver failure (ALF). Hepatocyte therapy is limited by several factors including limited homing of transplanted cells and liver functional improvement. Since beneficial effects of monounsaturated fatty acids on liver function and metabolism have been reported previously, our aim was to study oleic acid effects on hepatocyte transplantation outcome. Methodology: ALF was induced by acetaminophen (APAP) injection. Hepatocytes were isolated from male rats and transplanted intraperitoneally into female rats (ALF+HT group). Effect of oleic acid was assessed in rats fed an oleic acid rich diet (ALF+HT+OA group). Plasma levels of albumin (ALB), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) Alkaline phosphatase (ALP) were determined. Detection of Y-chromosome by PCR was used for homing assessment of transplanted hepatocytes. Finally, hematoxylin and eosin staining was used for histopathologic evaluation of liver. Results: APAP injection resulted in an increase in levels of ALT, AST and ALP. ALT level was decreased to normal range only in ALF+HT+OA group. Oleic acid administration lowered the maximum amount of elevated AST levels compared to ALF+HT group. No significant difference was observed between ALF+HT group and ALF+HT+OA group in ALP recovery. Plasma level of ALB was decreased after APAP injection which was only fully retrieved in ALF+HT+OA group. SRY detection by PCR confirmed successful engraftment of transplanted hepatocytes. H&E staining revealed that OA administration lead to an increase in the number of normal hepatocytes and reduced inflammation in the liver. Conclusion: In conclusion, our findings suggest that dietary oleic acid may improve hepatocyte transplantation success via improvement of liver function.
RESUMO
The disabled people are a part of our society [1%] and a disabled will face incorrect behaviors from childhood that is caused by an incomplete understanding of the society, but family can play a constructive role to increase the disabled abilities. This study was conducted to determine the views of deaf, blind and physical motor handicaps and their families regarding disability in the family in the Qazvin and Karaj. One hundred and fifty handicaps and their families from handicaps center, welfare office, the disabled school students, the Mostazafan and Janbazan foundation were enrolled in this study. Information was collected by using 2 questionnaires. T test and Analyses Of Variance have been applied for data analysis, using SPSS software. The results show that among different disabilities, the deaf ones and their families had more positive view than other disabled ones about their disabilities. Also, increasing the number of family members can cause more negative view about disability [p<0.05]. This study shows that there isn't a significant relationship between the disabled view and his / her gender, cause of disability and parents high education. With regard to the similarity of the disables and their families views about disability and also their need to obtain more awareness about disability, the disable's needs and complications, it is necessary to stress on correct training of disabled person, his/ her family and the society