Physiological effect of peri winkle [C. roseus] on diabetic albino rat

Diabetes mellitus [DM] is a major health problem worldwide in recent time. Asia and Africa are the most viable areas where the disease is feared to raise 2-3 folds. Many herbal products have been recommended for the treatment of DM in ancient literature of Ayurveda in India and other worldwide. Thirty male adult albino rats were used to investigate the effect of Catharanthus rosea [C.roseus] on diabetic rats. Rats were divided into three equal groups, control, diabetic non treated and diabetic C.roseus treated groups. After thirty days of treatment all rats of each group were sacrificed. The body weight of each rat was determined at the beginning and the end of each period. Blood glucose, serum insulin, lipid and protein profiles, liver and kidney functions, blood picture and liver glycogen were determined for each rat at the end of each period. Pancreatic samples were obtained and processed for microscopic and quantitative evaluation after staining the prepared sections with heamatoxylin and eosin as well as special stain for demonstration of the different pancreatic cells in the islets of Langerhans. The obtained results showed that the diabetic rats were diagnosed by laboratory assessment to body weight loss, hyperglycemia, and hypoinsulinemia, significant increase in liver and kidney functions, lipid and protein profiles and decreased liver glycogen content. While, C.roseus treatment led to a significant improvement in these parameters except liver function. Microscopically there was definite vaculation, degeneration, karyolysis and pyknosis of beta pancreatic cells in the diabetic group, while other pancreatic cells were not affected [alpha and delta cells]. The use of C.roseus treatment of this study greatly improves such cellular changes. It was recommended that the use of the water extract of C. roseus levies as a hypoglycemic agent may offer a new hope to the diabetics in future. It's well recommended to use variable doses and different periods of treatment to evaluate the best dose and period

Effect of Zingiber officinale on fatty liver induced by oxytetracycline in albino rats

Fatty liver causes were markedly increased in Egyptian people throughout last years. People prefer to use the medicinal plants instead of using chemical compounds because they are cheap and have few side effects compared to chemical compounds. Ginger is a natural dietary rhizome with antioxidative, anti-inflammatory, and anti-carcinogenic activities. The aim of this study was to evaluate the possible potential therapeutic and protective effects of Zingiber officinale [ginger] against oxytetracyclininduced fatty liver in an attempt to understand its mechanism of action, which may pave the way for possible therapeutic applications. Albino rats were divided into two major groups, 15 rats for each. The first group was divided into three sub-groups: a] control, b] fatty liver group; that was injected intraperitonealy with oxytetracycline [120mg/kg] for three consecutive days and c] ginger treated group; which was treated with ginger water extract [125 mg/kg] for 30 days after fatty liver induction . All animals were scarified after 33 days of the beginning of the experiment. The second group was divided into three subgroups: a] control, b] fatty liver group; that was injected intraperitonealy with oxytetracycline [120 mg/kg] for three consecutive days and c] ginger protective group; which received ginger for 15 days before induction of fatty liver, then sacrificed after induction of fatty liver [3 days]. Blood samples were collected for biochemical analysis. Liver specimens were obtained and fixed in 10% formalin for histological study. Fatty liver groups showed high significant increase in serum glucose, cholesterol, triglycerides, LDL cholesterol, ALAT, ASAT, GGT, LDH, urea, creatinine and A/G ratio while total protein, albumin, globulin and HDL cholesterol were significantly decreased compared to control group. These biochemical changes were accompanied with histopathological alterations in fatty liver tissue. The treatment with ginger ameliorated most of the evaluated biochemical parameters and improved the induced degenerative histopathological changes. The pre-treatment with ginger before the induction of fatty liver gave some protection against factors that experimentally induced fatty liver. Ginger as diet additive is recommend for fatty liver patients or those people who have hyperlipidemic family history

Effect of Cichorium intybus L. on fatty liver induced by oxytetracycline in albino rats

Fatty liver is now one of the most common diseases in Egypt. People prefer to use the medicinal plants instead of using chemical compounds because they are cheap and have few side effects compared to chemical compounds.The current investigation was carried out to examine the possible potential therapeutic and protective effects of Cichorium intybus [chicory] against oxytetracyclin-induced fatty liver in an attempt to understand its mechanism of action, which may pave the way for possible therapeutic applications. Albino rats were divided into two major groups, 15 rats for each. The first group was divided into three sub-groups: a] control, b] fatty liver group; that was injected intraperitonealy with oxytetracycline [120mg/kg] for three consecutive days resulting in steatosis and c] chicory treated group; which was treated with chicory water extract [70 mg/kg] for 30 days after fatty liver induction . All animals were scarified after 33 days of the beginning of the experiment. The second group was divided into three subgroups: a] control, b] fatty liver group; that was injected intraperitonealy with oxytetracycline [120mg/kg] for three consecutive days and c] drug protection group; which received chicory for 15 days before induction of fatty liver, then sacrificed after induction of fatty liver [3 days]. Blood samples were collected for biochemical analysis. Liver specimens were obtained and fixed in 10% formalin for histological study. Fatty liver groups showed high significant increase in serum glucose, cholesterol, triglycerides, LDL cholesterol, ALAT, ASAT, GGT, LDH, urea, creatinine and A/G ratio while total protein, albumin, globulin and HDL cholesterol were significantly decreased compared to control group. These biochemical changes were accompanied with histopathological alterations in fatty liver tissue. The treatment with chicory ameliorated most of the evaluated biochemical parameters and improved the induced degenerative histopathological changes. The pre-treatment with chicory before the induction of fatty liver gave some protection against factors that experimentally induced fatty liver. Chicory as diet additive is recommend for fatty liver patients or those people who have hyperlipidemic family history