Synovial sarcoma of the esophagus: a case report and review of literature

Synovial sarcoma is an uncommon soft tissue tumor occurring mainly in the periarticular region of the extremities in young adults. It happens less frequently in the head and neck, mediastinum, lungs, heart, and digestive tract

A 28-year-old man two months after total esophagectomy with final diagnosis of esophageal synovial sarcoma was referred to our Positron Emission Tomography [PET-CT] department for the evaluation of treatment response and further treatment planning. To our knowledge this case is the 11[th] case of esophageal synovial sarcoma, being reported in the literature

We presented the 11[th] case of esophageal synovial sarcoma. Synovial sarcomas are very rare tumor entities, particularly in the gastrointestinal tract and are likely to be mistaken with other more common tumors such as gastrointestinal stromal tumors

Asymptomatic giant lipoma of the right ventricular outflow tract: a case report

Cardiac lipomas are extremely rare tumors of the heart. They are usually symptomatic and rarely may be found incidentally in autopsies. Here we describe a 23-year-old healthy man, in whose physical examination for employment a murmur was found incidentally. Transthoracic and then transesophageal echocardiographic examination showed a 4-cm oval-shaped mass in the right ventricular outflow tract. He underwent elective surgery, during which the tumor was removed under cardiopulmonary bypass and aortic-cross clamping via right atriotomy. The postoperative course was uneventful, and the patient was in good condition at 1 year's follow-up

Multidrug-resistant tubercular appendicitis: report of a case

Acute tubercular appendicitis has remained a rare disease despite frequent cases of tuberculosis. The following study reports a patient with multidrug-resistant [MDR] pulmonary tuberculosis that developed acute appendicitis. Histopathology of the appendix was compatible with tuberculosis. The patient had a good outcome after surgery and medical therapy

Clonality of the immunoglobulin heavy chain genes in B cell non-Hodgkin lymphoma using semi-nested PCR

Identification of gene rearrangements and clonality analysis are important techniques for the diagnosis of malignant lymphoproliferative diseases. These methods have various sensitivities based on the type of primer used and method of determination of polymerase chain reaction [PCR] products. This study aimed at determining the clonality of B cell non-Hodgkin lymphoma in Iranian patients using PCR method and 2 primers of FR2 and FR3. Paraffin embedded blocks of 67 patients with B cell lymphoma and 19 cases with lymphoid hyperplasia of the lymph nodes who presented to NRITLD, Masih Daneshvari Hospital were retrospectively reviewed. After extracting the genomic DNA using phenol and chloroform, clonal analysis was performed using semi-nested PCR by using two primers: FR2 and FR3. PCR products were determined using 2 techniques of heteroduplex analysis, polyacrylamide gel and silver staining and the conventional method of agarose gel and ethidium bromide staining. Appearance of 1 or 2 bands in the desired location were considered as a sign of clonality. Monoclonal gene rearrangement was observed in 62 out of 67 patients [92.5%] as one or two discrete bands appeared within 60-120 base pairs [bp] and 200-300 bp range. Of the mentioned patients, 53 cases [79.1%] had FR2 and 51 [76.1%] had FR3 rearrangement. Heteroduplex analysis along with silver nitrate staining detected 3 out of the remaining 5 cases of lymphoma to be monoclonal. These cases had been reported negative by the conventional technique. In total, 65 out of 67 patients [97%] showed monoclonal gene rearrangement using both the abovementioned techniques. All hyperplasia cases were polyclonal by this method. Our study showed that evaluation and detection of clonality using PCR, FR2 and FR3 primers along with heteroduplex analysis is a rapid sensitive technique for the diagnosis of malignant lymphomas

Assessment of angiotensin-converting enzyme gene in idiopathic pulmonary fibrosis

Interstitial pulmonary fibrosis [IPF] is a progressive fibrotic interstitial lung disease with a distinct histopathological form referred to as usual interstitial pneumonia [UIP]. Evidence has indicated that a local renin-angiotensin system is present in distal lung parenchyma. Expression of the component of this system is present in a number of fibrotic lung diseases. In this study, we assessed the association of Insertion/Deletion [I/D] polymorphism of angiotensin-converting enzyme [ACE] gene in IPF. By using semi-nested PCR, we determined the I/D polymorphism of ACE gene in 23 paraffinembedded open lung biopsy specimens from patients having clinical and imaging findings of IPF and pathologic diagnosis of UIP at National Research Institute of Tuberculosis and Lung Disease [NRITLD]. Afterwards, we compared the results with I/D polymorphism of ACE gene in a healthy control group [n= 88]. The frequency of I allele was 71.7%[33 out of 46] and the frequency of D allele was 28.3% [13 out of 46]. The frequent genotype was I/D [56.5%] which was statistically significant comparing with healthy group [27.3%]. We had no D/D genotype .There was a difference in the distribution pattern of ACE genotype between patients and controls [P < 0.05]. Our study revealed an association between carriage of I allele and I/D genotype in IPF