Periodontal disease induced by porphyromonas gingivalis and fusobacterium nucleatum in Wistarrats / Periodontite induzida pela porphyromonas gingivalis e fusobacterium nucleatum em ratos Wistar

Prior studies have shown that it is necessary to place ligatures around molars to study periodontal destruction in rats. The present research aims to examine a periodontal disease model in which specific pathogen-free Wistar rats are orally exposed to Porphyromonas gingivalis associated with Fusobacterium nucleatum. Periodontitis was induced by specific infection with P. gingivalis and F. nucleatum. Twenty adult male Wistar rats were divided into two groups. The control animals were not infected. The experimental animals were repeatedly infected with Porphyromonas gingivalis and Fusobacterium nucleatum for one week. For the next three weeks, the animals were repeatedly infected with P. gingivalis alone. The distance from the cemento-enamel junction (CEJ) to the alveolar bone crest of the second molar was measured at different sites: buccal-distal (d), buccal-furcation region (f), buccal-mesial (h), and area region. The Mann-Whitney test was applied (p<0.001). The results showed that all values obtained were significantly greater in the infected group. Infected group values for the measures d, f, h, and area were 0.41 mm, 0.46 mm, 0.67mm, and 1.04 mm2, respectively, while in the control group, values for the measures d, f, h, and area were0.19 mm, 0.26 mm, 0.26 mm, 0.88 mm2, respectively. Our study showed that four weeks following infection with Porphyromonas gingivalis and Fusobacterium nucleatum bone loss in Wistar rats could be identified.

Avaliação da capacidade inibitória de crescimento de S. mutans de diferentes materiais restauradores / Evaluation of inhibitory growth of S. mutans on different restorative materials

A adesão de bactérias ao dente e aos materiais restauradores apresentam correlação positiva com a cárie e Doença Priodontal, portanto deve-se levar em consideração às características químicas e a textura de superfície do material restaurador à ser selecionado pelo profissional durante o ato operatório. O presente estudo avaliou o potencial de inibição de crescimento de S. mutans de diferentes materiais restauradores, contribuinbdo ao profissional para a seleção do material restaurador que envolva um menor acúmulo e crescimento desse tipo de bactéria. Foi medido em milímetros, a formação de halos de inibição de crescimento ao redor de discos de diferentes materiais restauradores, em meio de ágar-mitis salivarius com cepa de S. mutans, onde ficaram em estufa bacteriológica por 48 horas, em jarra de anaerobiose. O único material que inibiu o crescimento de S. mutans, no presente estudo, foi o amálgama dental de alto teor de cobre, ao contrário dos cimentos de ionômero de vidro convencional e reforçado com resina, e as resinas compostas