RESUMO
This study evaluated the effect of antiasthmatic medication on the surface roughness and color stability of dental restorative materials. A total of 60 disc-shaped specimens were fabricated from glass ionomer [n = 20], composite resin [n = 20] and feldspathic porcelain [n = 20]. Each material group was randomly divided into two subgroups [n = 10]: [1] control group and [2] test group. Control groups of the specimens were kept in artificial saliva. Test groups were exposed to salbutamol sulfate [Ventolin Nebules] using an inhaler machine. Surface roughness measurements were done using a profilometry and color measurements were done with digital colorimetry at baseline and after inhalation and storing in artificial saliva. A two-way analysis of variance [ANOVA] and post-hoc Fisher's least significant difference test were used to compare the change in surface roughness and color. The confidence level was set at 95%. Inhaler treatment significantly increased the surface roughness and color change of glass ionomer and composite resin materials [p < 0.05], while the surface roughness and color of feldspathic porcelain was not changed after inhaler treatment [p > 0.05]. The antiasthmatic inhaler medication [salbutamol sulfate] affected the surface roughness and color of composite resin and glass ionomer restorative materials
RESUMO
The purpose of this study was to investigate the ultrastructural effects of lead on the kidney cortex of rats. Wistar Albino rats (180-200g body weight) were divided into a controlled and lead acetate-exposed group. Rats received lead acetate at 500 ppm in their drinking water for 60 days. Both groups were fed with the same standard food, but lead acetate was added to the drinking water. During the experimental period, blood samples were taken from the abdominal aorta of the anesthetised animals. At the end of exposure, body weight and blood lead levels were measured. The kidney tissue samples were prepared and analyzed by light and transmission electron microscopy. Cortical renal tubules show various degenerative changes with focal tubular necrosis invaded by inflammatory cells. The ultrastructural alterations found in lead acetate-treated rats were a diminution in the amount of filtration slits, increased fusion of foot processes in epithelial cells of the glomeruli, increase of lysosomal structures and pinocytic vesicles as well as large mitochondria in proximal tubule cells.
El propósito de este estudio fue investigar los efectos ultraestructurales del plomo en la corteza renal. Ratas Wistar albinas (180-200g de peso corporal) fueron divididas en grupo control y grupo experimental. Las ratas recibieron 500 ppm de acetato de plomo en el agua potable durante 60 días. Ambos grupos fueron alimentados con el mismo alimento estándar, pero acetato de plomo se le añadió al agua potable al grupo experimental. Durante el período experimental, se tomaron bajo anestesia muestras sanguíneas desde la parte abdominal de la aorta. Al final de la exposición, fueron medidos el peso corporal y los niveles de plomo en la sangre. Fueron preparadas las muestras de tejido renal y se analizaron mediante microscopía de luz y electrónica de transmisión. Los túbulos renales corticales mostraron varios cambios degenerativos con necrosis tubular focal invadida por células inflamatorias. Las alteraciones ultraestructurales encontradas en las ratas tratadas con acetato de plomo correspondieron a una disminución en la cantidad de ranuras de filtración, aumento de la fusión de los procesos podales en las células epiteliales de los glomérulos, aumento de la estructura lisosomal y las vesículas pinocíticas, así como grandes mitocondrias en las células del túbulo proximal.