Topical Moltkia coerulea hydroethanolic extract accelerates the repair of excision wound in a rat model / 中华创伤杂志(英文版)

<p><b>PURPOSE</b>To evaluate the effect of a hydroethanolic extract of Moltkia coerulea ointment (MCO) on the healing of excision wound in a rat model.</p><p><b>METHODS</b>Circular surgical full thickness excision wound, with 314 mm² size, was induced in the anterior-dorsal side of each rat. Three different doses of MCO (1%, 3% and 6%) were administrated. On Day 3, 7, 14 and 21, the tissue was sampled and immune cells, fibroblasts and fibrocytes distribution per one mm² of wound area, collagen density and re-epithelialization were analyzed. Moreover, the total flavnoid, phenols and anti-oxidant potential of the MCO were evaluated. Ultimately, the percentage of wound contraction in different groups was compared with each other.</p><p><b>RESULTS</b>Hydroethanolic extract of MCO significantly (p < 0.05) increased wound contraction percentage. The animals in medium and high dose MCO-treated groups exhibited remarkably (p<0.05) higher fibroblast and fibrocyte distribution and significantly (p < 0.05) lower immune cells infiltration. On Day 7 after injury, MCO up-regulated neovascularization in a dose-dependent way.</p><p><b>CONCLUSION</b>Our data showed that MCO shortened the inflammation phase by provoking the fibroblast proliferation. Moreover, MCO promoted the healing process by up-regulating the angiogenesis and provoking the structural cells proliferation as well as increasing the collagen synthesis, cross-linking, and deposition.</p>

[ effect of 4-week swimming training on rat's knee osteoarthritis induced by monosodium iodoacetate]

Osteoarthritis is the most common form of synovial joint diseases which affect old people all around the world and can lead to chronic pain and severe disability. The aim of present study was to investigate the therapeutic effect of swimming exercise on MIA-induced osteoarthritis in male rats' knee joint. Eighteen Wistar male rats [weight of 173 +/- 1 g, 8 weeks old] were randomly divided into 3 experimental groups: intact control, monosodium Iodoacetate [MIA] only and swimming. Intra-articular injection of MIA [50 microl] was applied on rats' right knee, similarly saline was injected in left knees. Training program used in the study was swimming with moderate intensity for 28 days. After the completion of the protocol, rats were killed and both knees of the animals were assessed histopathologically. One-way ANOVA [p<0/05] and post-hoc Tukey test were used for statistical analysis of data. Results indicated that moderate swimming training for 4 weeks significantly improved osteoarthritic symptoms of rats' knee in 3 histopathological measures of Depth Ratio of Lesions [p=0/001], Total Degeneration Width [p=0/001] and Significant Degeneration Width [P=0/001]. The findings of the present study showed that a moderate swimming training program exerts a beneficial influence on cartilage lesions induced by monosodium iodo acetate injection. Thus, the training protocol used in this study can be recommended for the treatment of osteoarthritis