RESUMO
Vancomycin-resistance among Enterococci has been escalating in recent years in many countries. Linezolid, an oxazolidinone is one of the novel drug that promised effective therapy of infections caused by vancomycin-resistant Enterococci [VRE].However, like with most of the previous antimicrobial agents, the clinical benefit of linezolid is being threatened by the emergence of resistant strains of MRSA and vancomycin resistant enterococci VRE, being reported in many countries. This study was conducted to establish linezolid susceptibility of VRE isolates by determining the in vitro activity of linezolid against vancomycin resistant Enterococci, isolated in our setup using fifty VRE isolates. The data collected from this study conclude that the vancomycin resistant Enterococci are 100% linezolid susceptible and presently there is no significant resistance against this unique oxazolidinone in our setup
RESUMO
Objective: To determine the frequency of urinary tract infection [UTI] in diabetes mellitus patients
Methodology: This was a cross sectional study, conducted from October to December 2017 at department of Microbiology, Sheikh Zayed Medical College/Hospital, Rahim Yar Khan. A total of 200 samples of urine, were collected from outdoor patient department of diagnosed diabetic patients and were inoculated on a CLED agar. These cultures plates were placed in incubator at 35-37 degree C in aerobic environment. Positive cultures having growth were identified by their colony characteristics, appearance and confirmed by biochemical tests. Pure culture was obtained and inoculated on Nutrient agar plate and discs of amoxicillin clavulanic acid [20Mug: 10Mug], ciprofloxacin [5Mug], imipenem [10 Mug], piperacillin tazobactam [10Mug] and gentamicin [10Mug] were applied. Plates were examined to read the zones of inhibition according to CLSI. Data was analyzed by using SPSS Version 20
Results: Uropathogens were isolated from 200 urine samples. Escherichia coli was the most frequent isolate [52%], followed by Klebsiella species [17%], Proteus species [8%], Pseudomonas aeruginosa [6%] and Staphylococcus species[17%]. Gram positive bacteria were highly sensitive to imipenem, Sulzone [Cefoperazone/Sulbactam]. Gram negative bacteria were sensitive to imipenem, sulzone, Fosfomycin, ceftriaxone, Ciprofloxacin and resistant to amoxicillin clavulanic acid, nitrofurantoin, piprracillin tazobactam and gentamicin
Conclusion: DM patients are prone to Urinary Treat Infection and should carefully be treated after culture and sensitivity, as rational use of antibiotics make it difficult to treat. Microbial Drug Resistance against uropathogens is a major problem and this problem should be properly evaluated