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1.
Asian Pac J Allergy Immunol ; 2004 Jun-Sep; 22(2-3): 159-63
Artigo em Inglês | IMSEAR | ID: sea-37112

RESUMO

The etiology of systemic lupus erythematosus (SLE) is unknown but genetic factors seem to play a role in the disease pathogenesis. The tumor necrosis factor alpha (TNFa) gene, encoded at the TNF locus in the MHC class III region, is now known to be an important candidate gene in SLE, due to the proinflammatory activities of the TNFa. The objectives of this study were to examine the role of the TNFa polymorphism for the susceptibility of Malaysian Chinese lupus patients to SLE and to determine its association with organ involvement. The allelic frequencies of the TNFa polymorphic variant (TNF2) of seventy lupus patients were determined during follow-up at the Medical Clinic of the National University Hospital Malaysia by PCR-RFLP technique. Sixty-four females and 6 males with a mean age of 33+/-12 years were included. Clinical data were obtained from case records. Autoantibody levels were measured by ELISA. Fifty-nine ethnically-matched blood donors were used as controls. The allelic frequency of the TNF2 variant was found to be significantly increased in the patients compared to the controls (52.8% vs 33.8%). SLE patients with the polymorphic TNF2 variant were found to be at increased risk of central nervous system involvement (p = 0.004, RR = 2.59) and to have an increased frequency of anti-La antibodies (p = 0.03). In view of these findings we suggest that TNF2 variant is playing a role in conferring susceptibility to SLE and in the disease pathogenesis.


Assuntos
Adulto , Feminino , Frequência do Gene , Predisposição Genética para Doença , Humanos , Lúpus Eritematoso Sistêmico/genética , Malásia , Masculino , Reação em Cadeia da Polimerase , Polimorfismo Genético , Polimorfismo de Fragmento de Restrição , Fator de Necrose Tumoral alfa/genética
2.
Asian Pac J Allergy Immunol ; 2001 Jun; 19(2): 93-100
Artigo em Inglês | IMSEAR | ID: sea-36992

RESUMO

The frequency of the HLA class II antigens/alleles (HLA-DR, DQ and DP) were studied in 70 Malaysian Chinese patients with systemic lupus erythematosus (SLE) to examine the contribution of these genes to disease susceptibility, their clinical expression and Immunological responses. This was done using modified PCR-RFLP technique. These samples were then compared with 66 ethnically matched controls. We found a strong association of the DQA1*0102 (p corr = 0.032, rr = 3.39), DQB1*0501 (p corr = 0.003, rr = 4.55), *0601 (p corr = 0.006, rr = 4.22) and DPB1* 0901(p corr = 0.02, rr = 4.58) with SLE. Clinically, we found a strong association of DR2 and DQA1*0301 with renal involvement and DQA1*0102 with alopecia. Immunologically, statistical analysis (Chi-square test ) showed a strong association of DQA1*0102 with anti-Ro/La antibodies while DQA1*0301 was observed to be strongly associated with antibodies to ds DNA. DQA1*0102 was found more frequently in those with a later disease onset (30 years of age or above). From these data we suggest that the HLA class II genes play a role in conferring disease susceptibility and clinical and immunological expression.


Assuntos
Adolescente , Adulto , Idoso , Povo Asiático/genética , Autoanticorpos/genética , Feminino , Expressão Gênica , Frequência do Gene/genética , Predisposição Genética para Doença/etnologia , Antígenos HLA-DP/genética , Antígenos HLA-DQ/genética , Antígenos HLA-DR/genética , Antígenos de Histocompatibilidade Classe II/genética , Humanos , Lúpus Eritematoso Sistêmico/etnologia , Malásia/etnologia , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade
3.
Asian Pac J Allergy Immunol ; 1996 Dec; 14(2): 125-8
Artigo em Inglês | IMSEAR | ID: sea-36615

RESUMO

We studied the prevalence of antinuclear (ANA), anti-double stranded DNA (dsDNA), anti-Sm and anti-RNP antibodies in a group of 93 blood donors (age range: 18-58 years). Antinuclear and anti-ds DNA antibodies were detected by immunofluorescence (IF) using HEp2 cells and Crithidia luciliae as substrates, respectively, while anti-Sm and anti-RNP antibodies were assayed by ELISA. ANA was found in 6.5% while anti-dsDNA antibodies were not detected in any of the subjects. The 98th percentile was used as cut off where values greater than 0.651 for anti-Sm and 0.601 for anti-RNP antibodies were taken to be positive. This gives a frequency of 1.1% for both antibodies. There was no significant association of antibody positivity with sex or race. We conclude that certain autoantibodies are present in low titres in the normal Malaysian Individuals, at a different frequency compared to other studies probably due to genetic, ethic or environmental factors.


Assuntos
Adolescente , Adulto , Anticorpos Antinucleares/sangue , Povo Asiático , Autoantígenos/imunologia , Doadores de Sangue , China , Ensaio de Imunoadsorção Enzimática , Humanos , Malásia , Pessoa de Meia-Idade , Prevalência , Valores de Referência , Ribonucleoproteínas Nucleares Pequenas , Proteínas Centrais de snRNP
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