Estudio Genético de la raza bovina criolla Casanare de Colombia y su relación con otras razas / Genetic study of the Colombian criollo breed Casanare and its relationship with others breeds

Se estudió la variabilidad genética de la raza Criolla Casanare de Colombia y sus relaciones con poblaciones cruzadas Cebú x Criollo, Cebú puro y Pardo Alpino. Para ello, mediante amplificación por PCR, se han analizado 22 microsatélites en 79 individuos (54 Casanare; 14 Cebú, 8 cruzados y 3 de Pardo Alpino). El número medio de heterocigotos observados y el número medio de alelos por locus fueron mayores en la población Criolla Casanare que en el resto, con valores de 0,72 y próximos a 0,70, respectivamente. Sólo el 3,6 por ciento de la variabilidad observada pudiera ser debida a diferencias entre los distintos grupos raciales (Fst global= 0,03634; P<0,01). La matriz de distancias genéticas mostró más proximidad entre los Criollos Casanare y los cruzados, que entre éstos y los Cebú. Se constató la influencia del Cebú sobre la actual raza Casanare que se encuentra en la actualidad en grave peligro de extinción.

The genetic variability of the Creole Casanare breed of Colombia and its relations with crossed populations Zebu x Creole, pure Cebu and Brown Swiss was studied. For it, by means of PCR amplification, 22 microsatellites have been analyzed in 79 individuals (54 Creole Casanare; 14 Zebu, 8 crossed Zebu x Creole and 3 Brown Swiss). The average of heterozygotos observed and the average number of alleles per locus they were major in the population Creole Casanare that in the rest with values of 0.72 and close to 0.70 respectively. Only 3.6% of the observed variability it could be due to differences between the different racial groups (global FST = 0.03634; P< 0.01). The matrix of genetic distances showed more proximity between the Creole Casanare and the crossed ones that between these and Zebu. The influence of the Zebu on the current breed Casanare was stated, who is at present in serious danger of extinction.

Genetic characterization of the Spanish Trotter horse breed using microsatellite markers

To assist in selection schemes we carried out the first genetic characterization of the Spanish Trotter horse (Trotador Español). We used 16 microsatellite markers to genotype 40 unrelated Spanish trotters, 25 native Balearic horses (11 Menorquina and 14 Mallorquina horses) and 32 Andalusian horses. The observed heterozygosity for the Spanish Trotters was 0.647 ± 0.037 and the expected heterozygosity was 0.696 ± 0.026 while the average number of alleles per locus was 6.0 ± 0.341, these values being similar to the data published for other horse breeds. We also tried to establish the importance of the Mallorquina and Menorquina breeds in the present Spanish Trotter population. Only 9 percent of the total genetic variability could be attributed to differences between breeds (mean F ST = 0.09 ± 0.010). Recent migration rates were confirmed the low recent genetic relationship between the Balearic breeds and Spanish Trotters, indicating that the genetic background of the present Spanish Trotter population is not based on the native Balearic horse population.