Bathymetric modeling from satellite imagery via Single Band Algorithm [SBA] and Principal Components Analysis [PCA] in southern Caspian Sea

Remotely sensed imagery is proving to be a useful tool to estimate water depths in coastal zones. Bathymetric algorithms attempt to isolate water attenuation and hence depth from other factors by using different combinations of spectral bands. In this research, images of absolute bathymetry using two different but related methods in a region in the southern Caspian Sea coasts has been produced. The first method used a Single Band Algorithm [SBA] and assumed a constant water attenuation coefficient throughout the blue band. The second method used Principal Components Analysis [PCA] to adjust for varying water attenuation coefficients without additional ground truth data. PCA method [r=-0.672394] appears to match our control points slightly better than single band algorithm [r=-0.645404]. It is clear that both methods can be used as rough estimates of bathymetry for many coastal zone studies in the southern Caspian Sea such as near shore fisheries, coastal erosion, water quality, recreation siting and so forth. The presented methodology can be considered as the first step toward mapping bathymetry in the southern Caspian Sea. Further research must investigate the determination of the nonlinear optimization techniques as well as the assessment of these models' performance in the study area

Molecular characterization of GTP binding protein gene in dermatophyte pathogen trichophyton rubrum

Trichophyton rubrum [T. rubrum] is an anthropophilic dermatophyte that is distributed worldwide and causes common cutaneous disease such as mycosis. Although several properties of this fungus have been investigated so far, however a few studies were carried out in the field of molecular biology of this fungus. In the present study we tried to identify its molecular characterization of the goanosin three phosphat [GTP] binding protein gene. Pairs of 21 nt primers were designed from highly conserved regions of the gene in other fungi. The primers were utilized in PCR by using isolated genomic DNA tem-plate as well as cytoplasmic RNA of T. rubrum and the PCR and RT-PCR fragments were then sequenced. About 645 nucleotides have been sequenced which encodes a polypeptide with 214 amino acids. Nucleotide sequence comparison in gene data banks [NCBI, NIH] for both the DNA and its deduced amino acid sequence revealed significant homology with GTP binding protein genes and proteins of other eukaryotic cells. The amino acid sequence of the encoded protein was about 64% identical to the sequence of GTP binding protein from other fungi. In summary, we have cloned the first GTP binding protein of dermatophytes and characterized it as a member of this gene family in other eukaryotic cells