Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)

The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed. (AU)

Gasteroid mycobiota of Rio Grande do Sul State, Brazil: Lysuraceae (Basidiomycota) / Micobiota gasteróide do Estado do Rio Grande do Sul, Brasil: Lysuraceae (Basidiomycota)

As part of a review of gasteroid mycobiota from Rio Grande do Sul State, in southern Brazil, members of the Lysuraceae (Phallales) family were studied. Fresh and herbarium specimens were analyzed macro- and micromorphologically. Lysurus cruciatus, L. cruciatus var. nanus (new record from Brazil) and L. periphragmoides have been collected in the area. Their specific limits, distribution and ecological data are discussed. Macroscopic photographs and line drawings of the basidiospores are presented

Como parte de um trabalho de revisão dos fungos gasteróides do Estado de Rio Grande do Sul, Brasil, a família Lysuraceae (Phallales) foi estudada. Espécimes recém-coletados e preservados em herbários foram estudados macro e micromorfologicamente. Lysurus cruciatus, L. cruciatus var. nanus (primeiro registro para o Brasil) e L. periphragmoides foram coletadas na área de estudo. Seus limites taxonômicos, ecologia e distribuição são discutidos. Fotos macroscópicas e ilustrações dos basidiósporos são apresentadas.