Detection of DNA damage in nonalcoholic steatohepatitis; and its correlation with serum imbalance of adiponectin/tumor necrosis factor-alpha

NASH is a potentially serious condition, since as many as 25% of patients with NASH may progress to cirrhosis and experience complications of portal hypertension, liver failure, and hepatocellular carcinoma. The aim of the present work is to study DNA damage in patients with NASH and correlate this damage with serum levels of adiponectin and TNF-alpha in those patients to evaluate their role in the pathogenesis of the disease. The study was carried out on 20 patients with NASH [8 males and 12 females] and 10 healthy age and sex matched individuals as a control group. Detection of DNA damage in liver tissues was performed by gel electrophoresis. Serum levels of adiponectin and TNF-alpha were estimated in all patients and control group. There was a statistically significant increase in DNA damage in NASH patients than control subjects, detected by the intensity of the damaged DNA bands expressed by maximum optical density [max. OD], where it was 36.57 +/- 18.60 in NASH group and 0.088 +/- 0.02 in control subjects [p<0.001]. The intensity of the damage and fragmentation of DNA was not the same in all patients with NASH. More damage was detected in subgroup A, the max. OD in this group was 66.834 +/- 7.21. There was significant decrease in serum adiponectin level and significant increase in serum TNF- a level in NASH patients than control subjects [5.69 +/- 1.49 micro g/ml versus 13.67 +/- 1.6 micro g/ml and 9.12 +/- 3.1 pico g/ml versus 2.7 +/- 1.4 pg/ml respectively]. TNF- a serum level in NASH patients had significant positive correlation with DNA damage in those patients [r = 0.46, p <0.001], while serum adiponectin level had negative correlation with DNA damage in NASH [r = - 0.63, p 0.001]. DNA damage occurs in NASH patients as a result of increased oxidative stress and altered cytokine metabolism in the form of increased TNF- alpha and decreased adiponectin levels. Measures to prevent oxidative stress or inhibit TNF-alpha or increase adiponectin may improve NASH

Correlation between lifestyle profile and health related indicators among middle age working women

This study was carried out to describe the lifestyle profile behavior of middle age working women, and examining the association between lifestyle behavior and the presence of health related chronic conditions. This study was carried on 125 non pregnant middle age [30-60 years] working women from 4 different work sites. Among the important studied issues of lifestyle practice assessed using a structured interview questionnaire that modified and revised by the researchers nutritional practice, physical exercise, rest and sleep practice, stress management, health care practice, and health perception. The tool also include other items about demographic characteristics, present health status and health status indicators [Body Mass Index [BMI], blood pressure, and blood analysis for blood glucose, cholesterol and hemoglobin that assessed by the researchers]. The data from the interviews were analyzed using SPSS, yielded frequencies, mean, standard deviation, pearson's product moment correlation and one way analysis of variance [ANOVA]. The findings of this study revealed that the mean age of women was 41.01 +/- 6. The practice of physical exercise was the lowest lower score, only .8% practicing regular exercise, and 68.7% have moderate perception of their health, even the healthy women. Also, a positive correlation was found between higher nutritional score and practicing exercise. About 36.8% have chronic condition, 16% of conditions are detected and referred. WHO categorization of BMI [normal < 25, overweight > or = 25 < 30, obese > or = 30 < 39, morbidly obese > or = 39] was used to assess obesity, 75.2% of participants were found to be obese or morbidly obese, the mean BMI was 33.29 +/- 5.08. There was a positive correlation between BMI and elevated systolic and diastolic blood pressure. Also a positive correlation between elevated systolic and diastolic blood pressure and high cholesterol level. In conclusion, lifestyle practice continues to have a significant impact on women's health status. Nursing interventions are essential to enhance health lifestyle practice among middle age working women