Alpha-glucosidase inhibitory active constituents contained in nutshell of Trapa acornis / 中国中药杂志

<p><b>OBJECTIVE</b>To study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase.</p><p><b>METHOD</b>EtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques.</p><p><b>RESULT</b>Nine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase.</p><p><b>CONCLUSION</b>All compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.</p>

Effect of lacidophilus exopolysaccharides on immunity / 中国药理学通报

Aim To study the effect of Lacidophilus exopolysaccharides(LAEPS) on immunity. Methods Effects of LAEPS on immunity were investigated by delayed type hypersensitivity reaction, haemolytic plaque assay and macrophage function assay in mice. Results LAEPS ip 7～8 day promoted delayed type hypersensitivity reaction, increased haemolytic plaque and enhanced macrophage function in a dose-dependent manner.Conclusion LAEPS is able to enhances immunity.