RESUMO
Objective:To investigate the expression of intestinal alkaline phosphatase (IAP) in intestinal mucosa with bile deficiency and the effect of bile on the expression of IAP in intestinal epithelial Caco-2 cell model.Methods:Thirty healthy male SD rats were randomly divided into control group (Ctrl, n=10), external drainage group (ED, n=10) and obstructive jaundice group (OJ, n=10). Ileum specimens were collected on the 7th day after modeling. Western blot and immunohistochemical staining were used to determine the expression of IAP in rat intestinal mucosa. Different concentrations of human bile were used to treat on Caco-2 cells, and Western blot was used to detect the changes in IAP expression in Caco-2 cells. Results:Rat models were successfully established. The expression level of IAP in the intestinal mucosa of ED group [(9.19±1.67)%] was significantly lower than that of the Ctrl group [(15.09±0.61)%, P<0.05]; the expression of IAP in the intestinal mucosa of OJ group [(6.86±1.07)%] was significantly lower than that of the Ctrl group ( P<0.05). Through in vitro cell experiments, expression of IAP in Caco-2 cells was increased in a time and dose-dependent manner when treated with human bile. Conclusions:Bile deficiency in the intestine can cause inhibition of IAP in the intestinal mucosa. Bile can promote the expression of IAP in intestinal mucosal epithelial cells.
RESUMO
Bile acids are the end product of cholesterol metabolism,which is the main component of bile.Bile acids can not only promote the absorption of fat and fat-soluble vitamins but also be used as the important signal molecules to activate nuclear receptors.It regulates metabolism of bile acids and intestinal homeostasis.The bile acids' effect on intestinal mucosal barrier function has been controversial until now.Bile acids is both hydrophilic and hydrophobic simultaneously.Hydrophilic bile acid can promote cell proliferation,while hydrophobic bile acid can promote cell apoptosis.The stronger the hydrophobicity is,the greater the cellular damage effect will be.The activation of nuclear receptor by bile acids can protect intestinal mucosal barrier.New research progress of the bile acid regulation in intestinal mucosal barrier function is reviewed in this article.